LOCUS       sequence-136344-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a toehold mutant of the circRAJ31 riboregulator encased in
            a permuted intron exon ribozyme, which circularises the 
            riboregulator. This mutant does not activate cisRAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: circRAJ31-Toehold_mutant
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(81..112)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     160..176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     160..176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    728..746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    753..771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
       61 aacctcttac gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta
      121 tggaggtcag gtatgattta aatggtcagt attgagcctc aggaaacagc tatgacatca
      181 agctgactag ataatctagc tgatcgtgga ccgatcatac gtataatgcc gtaagatcac
      241 gggtcggacg acagctcgct cgtccagaac tgatccagtc gcgctgctct cgtcgatccc
      301 tgcagaaaaa aaatccttag ctttcgctaa ggatcaattg agtcctacaa tttagcacgg
      361 gattgtctac tagagaggtt ccccgtttag atagattaca agtataagtc accttatact
      421 caggcctcaa ttaacccaag aaaacatgct agcagaccaa tacagtgaca gcggataggg
      481 acttaattgc ggatggggtg tcggtaaagc ttaatattga tcccctagga atattaaacg
      541 gtagcattat gttcagataa ggtcgttaat cttaccccgg aattatatcc agctgcatgt
      601 caccatgcag agcagactat atctccaact tgttaaagca agttgtctat cgtttcgagt
      661 cacttgaccc tactccccaa agggatagtc gttaggcatt tatgtagaac ctctagagtg
      721 ctcagtatct ctatcactga tagggatgtc aatctctatc actgataggg agaattcggt
      781 gcgagcggat cgagcagtgt cgatctgtcg cc
//
