LOCUS       sequence-136345-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taRAJ31min, a linear riboregulator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: taRAJ31min
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(66..97)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     145..161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     145..161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    425..443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
       61 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
      121 atttaaatgg tcagtattga gcctcaggaa acagctatga catcaagctg actagataat
      181 ctagctgatc gtggaccgat catacgtata atgccgtaag atcacgggtc ggacgacagc
      241 tcgctcgtcc agaactgatc cagtcgcgct gctctcgtcg atccctgcag aaaaaaaatc
      301 cttagctttc gctaaggata gaccaataca gtgacagcgg atagggactt aattgcggat
      361 gagacgacgg taaagcttaa tattgatccg tgctcagtat ctctatcact gatagggatg
      421 tcaatctcta tcactgatag ggagaattcg gtgcgagcgg atcgagcagt gtcgatctgt
      481 cgcccgtctc aaacgcaacc ctccggcggt cgcatatcat tcaggacgag cctcagactc
      541 cagcgtaact ggactgcaat caactcactg gctcaccttc cggtccacga tcagctagaa
      601 tcaagctgac tagataaact ggccgtcgtt t
//
