LOCUS       sequence-136346-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA against CRTC3 (3rd generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Jeanneteau FD, Lambert WM, Ismaili N, Bath KG, Lee FS, Garabedian 
            MJ, Chao MV
  TITLE     BDNF and glucocorticoids regulate corticotrophin-releasing hormone 
            (CRH) homeostasis in the hypothalamus.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1305-10. doi: 
            10.1073/pnas.1114122109. Epub 2012 Jan 9.
  PUBMED    22232675
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1114122109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-24- 24"; volume: "109"; issue: "4"; pages: "1305-10"
COMMENT     Sequence Label: pLL3.7 shRNA against CRTC3
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     47..63
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        254..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gatcgctagt tctctagcag aatcagaatt ggtctttttg aattctcgag gtcgacggta
       61 tcgataagct cgcttcacga gattccagca ggtcgaggga cctaataact tcgtatagca
      121 tacattatac gaagttatat taagggttcc aagcttaagc ggccgcgtgg ataaccgtat
      181 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      721 gcggtaggcg tgtacggtgg
//
