LOCUS       sequence-136354-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..206
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(259..278)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     313..332
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(641..661)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg
       61 gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt
      121 tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc
      181 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacact agtggatcca
      241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      421 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
      481 tcaaggcact atacatcaaa tatttttcca taattttctt gtatagcagt gcagcttttt
      541 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
      601 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
      661 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
      721 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
      781 aggt
//
