LOCUS       sequence-136355-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    142..211
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    240..374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(264..283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     318..337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(646..666)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcacccgcc gacgccgtcg cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt
       61 cgagggcgag ggcttcccgg tgcgccgcgc gttcgccggg atcaactacc gccacctcga
      121 cccgttcatc atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaggg
      181 cacgccctgg cacccgcacc gcggcttcga gaccgtgacc tacatcgtcg acactagtgg
      241 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      301 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      361 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      421 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      481 tctagtcaag gcactataca tcaaatattt ttccataatt ttcttgtata gcagtgcagc
      541 tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg
      601 actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt
      661 ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc
      721 ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt
      781 tccataggtt ggaatctaa
//
