LOCUS       sequence-136357-        9707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 5, isoform 1 
            protein (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to alkaline phosphatase + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn5.1-AP-His
FEATURES             Location/Qualifiers
     source          1..9707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6287..6304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6330..6451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6367..6386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6421..6440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6489..6506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6660..7248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6740..6759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7419..8210,8211..8279))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8042..8061
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8280..8384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8452..8470
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8508..8530)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8532..8552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8537..8867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8718..8853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8780..8799
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9004..9023
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9090..9545)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9227..9248)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9439..9458
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9671..9693
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9685..9702
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9686..9702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcttcctg
     1621 ttggagactc attctgttcc tgtctgtcac caggtggctt tcagattact ctgaagctct
     1681 ctctggcctt tctacttcat atgctgcttt gctcagaatt aagaagagtt caacctcacc
     1741 ctttggttcc aagtccagac cacggttcag cagtccttca ttaggtacca tcagtgtttc
     1801 tccaccaagc tggcgagggg cagctcagca gtaccactcc ccaggcaacc tttatcactc
     1861 ctcagatgcc ttcagacaag atgaaagtgt ggattatggg ccagtatttg tgcaagaacc
     1921 tgatgatgtc atttttccaa ctgattctga tgaaaagaag gtagctctaa attgtgaagt
     1981 tcgtggcaac ccatctccta gttacagatg gcttcggaat ggaactgaaa tagctctgga
     2041 gagtgactat cgctacagtc tgatagacgg caccttcatc atcagcaatc caagtgaact
     2101 cagggattct ggcctgtacc agtgcttagc caccaactct tttggaagta ttcttagtcg
     2161 agaagccact cttcagtttg cctatctggg aaattttagt ggccggacaa gaagtgcagt
     2221 ctcagtgcgg gaaggccagg gggttgttct gatgtgctct ccaccaccac attctccaga
     2281 gatcatctat agctgggtct tcaatgagtt cccttccttt gtggcagagg acagccggcg
     2341 gttcatatcc caagagacag gcaaccttta tatttctaaa gtgcaaacat ccgatgttgg
     2401 cagctacatt tgtctggtga aaaatgctgt gacaaatgct cgcgtgctca gtcctccaac
     2461 cccactcacg ctgcgaaatg atggtgtgat gggagaatat gagccaaaaa ttgaggtcca
     2521 ttttcctttc actgtcacag ctgctaaggg aacaactgtg aagatggaat gctttgcact
     2581 tggcaacccg gttccaacaa ttacttggat gaaggttaac ggttacattc ctagtaagtc
     2641 cagactgcgg aaatcccagg cagtgctgga gattccaaac ctgcagctgg atgatgccgg
     2701 aatatacgaa tgcacagctg aaaactcccg tggaaaaaac tcctttcgtg gacagctaca
     2761 aatattcaca tacccacact gggtacagaa actgaatgat acccagctgg acagtgggag
     2821 tcctctgcag tgggaatgca aggctactgg aaagcccaga cccacatacc gctggctgaa
     2881 gaatggagca cccctcttgc cgcagagcag agtggacaca gtgaatggaa tattggcgat
     2941 ccagagtgtg aatcaatcag atgctgggat gtatcagtgt ttggctgaga ataaatatgg
     3001 agctatttat gcgagtgctg agctgaagat tctagcttcg ccgccctcct tcgaactgaa
     3061 tcaagtgaag aaatccataa ttgttaccaa agaccgggga gtgctcatag agtgcgaacc
     3121 ccagggatct ccaaagccag ccatctcctg gaggaaggga gacaaggcag tgagggcgaa
     3181 taaaagaata gctattcttc cagacggaag cctgaggatc ctaaacgctt ctaaagcaga
     3241 tgaggggaag tacatttgcc aaggggtgaa catcttcggt tctgcagaga ttatagcttc
     3301 cctgtctgtc aaagaaccca caaggataga gcttactcct aaaagaacag agttaactgt
     3361 tggagagagc attgtcctca actgtaaagc tatccacgac gccagtttgg acgtcacttt
     3421 ctactggaca ttaaaaggac aacctattga ttttgagaaa gaaggcggac attttgaaaa
     3481 catcagggct caagcatcct ctgcagattt aatgatcagg aacatccttc tgatgcatgc
     3541 tgggagatat ggctgcaggg tacagaccac agcagacagt gtgtcagatg aggcagaact
     3601 tcttgttagg ggtcccccag ggccacctgg agtggtcata gtagaggaga ttacagaaag
     3661 cacagccact ctctcatgga gtccagcaac tgacaaccat agccccatct cttcttacaa
     3721 ccttcaggct cgcagcccct tctccctggg ctggcaaaca gtgaagacag tcccagaagt
     3781 catcacagga gacatggagt cagcaatggc tgtggaccta aacccctggg tagaatatga
     3841 attccgagtg gtggccacca atccaattgg gacaggagat ccaagcattc catctcgaat
     3901 gatccgcaca aatgaggcag ttccaaagac ggcacccagc aacgtgagtg gaagaagtgg
     3961 aagaaggcat gagttggtca ttgcctggga gccagtatca gaagagtttc agaatgggga
     4021 aggcttcggc tacatagtgg cattcagacc taatggaaca cgtggctgga aggagaaaat
     4081 ggttacgtct tcggaggcgt ccaagttcat ttaccgagat gaaagtgtcc ctcctctcac
     4141 accctttgag gtcaaagttg gtgtttataa taataaaggt gatgggcctt tcagccagat
     4201 cgtagtcatc tgttcagctg agggagaacc cactgctgct cccactgacg tcacagcaac
     4261 cagtgtgtca gtgtcagaga tttttgttgt atggaaacat gttaaggaga gtcttggaag
     4321 accacagggg tttgagatta gttactggaa agataccgag ccagaagact ccgtagaaac
     4381 agtccgaacc agagggaatg agtcattcgt catgttgacg ggattggaag gcaacacact
     4441 ctatcacctc acagtcaggg cttacaatgg agccggctat gggccaccca gcagggaagc
     4501 aagcaccacc acaaagaggc acccccctag ggagccacct ggcaacctcc ggtgggagca
     4561 gcagggctcc caagtttccc taggctggga gccagtcaga cccctagcca atgagtctga
     4621 agtcatgggt tacaaggttt tttataggca agagggtcac agcgaaggtc aagttattga
     4681 gacacagaaa cctcaagctg tagtgcctct acctgaggct ggagtctaca ttattgaagt
     4741 tcgagcgtac agtgagggag gagatggaac agctagctcc caaatcaggg ttccttcata
     4801 tactagtgga tcttccggaa tcatcccagt tgaggaggag aacccggact tctggaaccg
     4861 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     4921 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     4981 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     5041 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     5101 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     5161 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     5221 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     5281 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     5341 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     5401 ggacattgac gtgatcctag gtgggggccg aaagtacatg tttcgcatgg gaaccccaga
     5461 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     5521 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat
     5581 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat
     5641 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     5701 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     5761 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     5821 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     5881 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     5941 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     6001 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     6061 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     6121 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     6181 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     6241 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtcatc atcaccatca
     6301 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     6481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8401 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8461 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8521 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     8581 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8641 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8701 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8761 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8821 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8881 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8941 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9001 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9061 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     9121 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     9181 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     9241 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     9301 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     9361 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     9421 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     9481 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     9541 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9601 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9661 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
