LOCUS       sequence-136366-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E165 Hs.PIK3CB
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..143
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             354..362
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aaacgacggc cagtcttaaa attcgagctc ggtccgagga tccgaaataa tgattttatt
       61 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc
      121 aactttgtac aaaaaagttg gcaccatgtg cttcagtttc ataatgcctc ctgctatggc
      181 agacatcctt gacatctggg cggtggattc acagatagca tctgatggct ccatacctgt
      241 ggatttcctt ttgcccactg ggatttatat ccagttggag gtacctcggg aagctaccat
      301 ttcttatatt aagcagatgt tatggaagca agttcacaat tacccaatgt tcaacctcct
      361 tatggatatt gactcctata tgtttgcatg tgtgaatcag actgctgtat atgaggagct
      421 tgaagatgaa acacgaagac tctgtgatgt cagacctttt cttccagttc tcaaattagt
      481 gacaagaagt tgtgacccag gggaaaaatt agactcaaaa attggagtcc ttataggaaa
      541 aggtctgcat gaatttgatt ccttgaagga tcctgaagta aatgaatttc gaagaaaaat
      601 gcgcaaattc agcgaggaaa aaatcctgtc acttgtggga ttgtcttgga tggactggct
      661 aaaacaaaca tatccaccag agcatgaacc atccatccct gaaaacttag aagataaact
      721 ttatggggga aagctcatcg tagctgttca ttttgaaaac tgccaggacg tgtttagctt
      781 tcaagtgtct cctaatatga atcctatcaa agtaaatgaa ttggcaatcc aaa
//
