LOCUS       sequence-136390-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of EGFP in yeast. Has dual-mode promoter with 
            activation and repression capability for tuning gene expression in 
            yeast. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Mazumder M, McMillen DR
  TITLE     Design and characterization of a dual-mode promoter with activation 
            and repression capability for tuning gene expression in yeast.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9514-22. doi: 10.1093/nar/gku651.
            Epub 2014 Jul 23.
  PUBMED    25056312
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku651"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9514-22"
COMMENT     Sequence Label: pLAREG
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattgggaca acaccagtga ataattcttc acctttagac attattaatt tagtgtgtgt
       61 atttgtgttt gcgtgtctat agaagtatag taatttatgc tacaaaggac ctaatgtata
      121 aggaaagaat atttagagaa aagtgtgtga aattgttatc cgctcacaat tccacacaaa
      181 gaaaacaaga gttttatata catacagagc acatgcctcg aggtcgaccc cgggaaaaag
      241 aacaccctgt accagacaaa aagaacaccc tgtaccagac gtcgacaaaa agaacaccct
      301 gtaccagaca aaaagaacac cctgtaccag acaaaaagaa caccctgtac cagacggatc
      361 tactagtgcg gccgcatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
      421 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
      481 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
      541 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
      601 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
      661 ctatctactg gagaatatac taagg
//
