LOCUS       sequence-136405-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator. Under control of a 
            T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: pT7_circRAJ31
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..62)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     71..90
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        71..89
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      474..521
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      528..586
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(610..627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
       61 tctggaattc taatacgact cactataggg tctagaggtt ctacataaat gcctaacgac
      121 tatccctttg gggagtaggg tcaagtgact cgaaacgata gacaacttgc tttaacaagt
      181 tggagatata gtctgctctg catggtgaca tgcagctgga tataattccg gggtaagatt
      241 aacgacctta tctgaacata atgctaccgt ttaatattcc taggggatca atattaagct
      301 ttaccgtcgt ctcatccgca attaagtccc tatccgctgt cactgtattg gtctgctagc
      361 atgttttctt gggttaattg aggcctgagt ataaggtgac ttatacttgt aatctatcta
      421 aacggggaac ctctctagta gacaatcccg tgctaaattg taggactcaa ttgctagcat
      481 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgcagtcc ggcaaaaaaa
      541 cggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
      601 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      721 catgtgagca aa
//
