LOCUS       sequence-136406-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter panel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15A-J23106-deGFP-T500 (128p)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        88..122
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             135..157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(194..215)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(455..474)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     802..823
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctgggtggg ctaacgatat ccgcctgatg cgtgaacgtg acggacgtaa ccaccgcgac
       61 atgtgtgtgc tgttccgctg ggcatgcttt acggctagct cagtcctagg tatagtgcta
      121 gcgctagcaa taattttgtt taactttaag aaggagatat accatggagc ttttcactgg
      181 cgttgttccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      241 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      361 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      601 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      661 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      721 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      781 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcta
      841 actcgagcaa agcccgccga aaggcgggct tttctgt
//
