LOCUS       sequence-136408-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter panel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15A-J23151-deGFP-T500 (129p)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(148..169)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     497..516
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     756..777
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             814..836
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(849..883)
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
ORIGIN
        1 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
       61 ctgggttgaa ggctctcaag ggcatcggtc gacacagaaa agcccgcctt tcggcgggct
      121 ttgctcgagt tagatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      181 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
      241 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      301 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      361 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      421 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      481 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      541 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      601 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      661 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      721 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      781 gggaacaacg ccagtgaaaa gctccatggt atatctcctt cttaaagtta aacaaaatta
      841 ttgctagcgc tagcattgta cctaggactg agctagccat caagcatgcc cagcggaaca
      901 gcacacacat gtcgcggtgg ttacgtccgt cacgttcacg catcag
//
