LOCUS       sequence-136409-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The second of the two RNA lassos required for the RNA chain 
            construction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: Lasso 2
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      153..200
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      216..274
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(298..315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(549..568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acacacgacg taagtcgtgg tacattacct ggtacaagcc ttgtcccttg aagagaacaa
       61 ctaacaacta acaacaacaa tcaactatta aacggtagac ccaaacatac aacaacaatc
      121 aacaatcaac caaggctgtc ctcgtcgata tcctagcata accccttggg gcctctaaac
      181 gggtcttgag gggttttttg agcctcttcc tgcagtccgg caaaaaaacg gcaaggtgtc
      241 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acag
//
