LOCUS       sequence-136410-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter panel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 85 to 119)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15A-J23100-deGFP-T500 (130p)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..119
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(191..212)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(452..471)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     799..820
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggtgggcta acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg
       61 tgtgtgctgt tccgctgggc atgcttgacg gctagctcag tcctaggtac agtgctagcg
      121 ctagcaataa ttttgtttaa ctttaagaag gagatatacc atggagcttt tcactggcgt
      181 tgttcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      241 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      301 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      361 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      421 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      481 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      541 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      601 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      661 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      721 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      781 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatctaact
      841 cgagcaaagc ccgccgaaag gcgggctttt ctgtgtcgac cgatgcc
//
