LOCUS       sequence-136428-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of EGFP in yeast. Has dual-mode promoter with 
            activation and repression capability for tuning gene expression in 
            yeast. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Mazumder M, McMillen DR
  TITLE     Design and characterization of a dual-mode promoter with activation 
            and repression capability for tuning gene expression in yeast.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9514-22. doi: 10.1093/nar/gku651.
            Epub 2014 Jul 23.
  PUBMED    25056312
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku651"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9514-22"
COMMENT     Sequence Label: pLAREG
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agatgcggcc gcactagtag atccgtctgg tacagggtgt tctttttgtc tggtacaggg
       61 tgttcttttt gtctggtaca gggtgttctt tttgtcgacg tctggtacag ggtgttcttt
      121 ttgtctggta cagggtgttc tttttcccgg ggtcgacctc gaggcatgtg ctctgtatgt
      181 atataaaact cttgttttct ttgtgtggaa ttgtgagcgg ataacaattt cacacacttt
      241 tctctaaata ttctttcctt atacattagg tcctttgtag cataaattac tatacttcta
      301 tagacacgca aacacaaata cacacactaa attaataatg tctaaaggtg aagaattatt
      361 cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc
      421 tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg
      481 tactactggt aaattgccag ttccatggcc aaccttagtc actactttcg gttatggtgt
      541 tcaatgtttt gcgagatacc cagatcatat gaaacaacat gactttttca agtctgccat
      601 gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac
      661 cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat taa
//
