LOCUS       sequence-136443-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator. Under control of a 
            T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: pT7_circRAJ31
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(45..92)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(476..495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(477..495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      504..547
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(551..569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(797..816)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agggcagggt ggtgacacct tgccgttttt ttgccggact gcagcaaaaa acccctcaag
       61 acccgtttag aggccccaag gggttatgct agcaattgag tcctacaatt tagcacggga
      121 ttgtctacta gagaggttcc ccgtttagat agattacaag tataagtcac cttatactca
      181 ggcctcaatt aacccaagaa aacatgctag cagaccaata cagtgacagc ggatagggac
      241 ttaattgcgg atgagacgac ggtaaagctt aatattgatc ccctaggaat attaaacggt
      301 agcattatgt tcagataagg tcgttaatct taccccggaa ttatatccag ctgcatgtca
      361 ccatgcagag cagactatat ctccaacttg ttaaagcaag ttgtctatcg tttcgagtca
      421 cttgacccta ctccccaaag ggatagtcgt taggcattta tgtagaacct ctagacccta
      481 tagtgagtcg tattagaatt ccagaaatca tccttagcga aagctaagga ttttttttat
      541 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      601 tcttagacgt caggtggcac tcgagttgat cgggcacgta agaggttcca actttcacca
      661 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
      721 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      781 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accag
//
