LOCUS       sequence-136444-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The second of the two RNA lassos required for the RNA chain 
            construction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: Lasso 2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(55..102)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(297..316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(298..316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      334..377
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(381..399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(627..646)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aagggcaggg tggtgacacc ttgccgtttt tttgccggac tgcaggaaga ggctcaaaaa
       61 acccctcaag acccgtttag aggccccaag gggttatgct aggatatcga cgaggacagc
      121 cttggttgat tgttgattgt tgttgtatgt ttgggtctac cgtttaatag ttgattgttg
      181 ttgttagttg ttagttgttc tcttcaaggg acaaggcttg taccaggtaa tgtaccacga
      241 cttacgtcgt gtgtttctct ggtaggcttc tctcgtcata cggacgagga cagcctccct
      301 atagtgagtc gtattaagcg tcttcgaatt ccagaaatca tccttagcga aagctaagga
      361 ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat
      421 aataatggtt tcttagacgt caggtggcac tcgagttgat cgggcacgta agaggttcca
      481 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
      541 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      601 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      661 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      721 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      781 gtatggcaat gaa
//
