LOCUS       sequence-136447-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Citrine reporter for transfer fxns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..767)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cccgactctc aaggacagaa aagcccgcct ttcggcgggc tttgagttca tcatttgtac
       61 aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa gaccatgtga
      121 tctctctttt cgtttggatc tttggataat ctagattgat aggataagta atggttgtct
      181 ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc agctaattga
      241 acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc attcttttgt
      301 ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt gtgacctaaa
      361 atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac taaggtatca
      421 ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa aaaaatagtt
      481 ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg tttcatatga
      541 tctgggtatc tagcaaaaca catcaaacca taacctaaag tagtgactaa ggttggccat
      601 ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc gtaagtagca
      661 tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc atctaattca
      721 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacattag aaaccctc
//
