LOCUS       sequence-136452-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct targeting the PIE-target sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: AL562-2
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(54..101)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(244..263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(245..263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      281..324
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(328..346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(574..593)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agggcagggt ggtgacacct tgccgttttt ttgccggact gcaggaagag gctcaaaaaa
       61 cccctcaaga cccgtttaga ggccccaagg ggttatgcta gtgcgcagac gaggacagcc
      121 ttggttgttg ttgttcttgt tgttgtttgg gtctaccgtt taatatgtac caggtaatgt
      181 accacgactt acgtcgtgtg tttctctggt aggcttctct cgtcatacgg acgaggacag
      241 cctccctata gtgagtcgta ttaagcgtct tcgaattcca gaaatcatcc ttagcgaaag
      301 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
      361 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
      421 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      481 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      541 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      601 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      661 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      721 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      781 tacaccgtt
//
