LOCUS       sequence-136453-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: cisRAJ31-cat
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             33..44
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    141..160
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      207..236
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      392..486
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gtatatccag tgattttttt ctccatatta tgtttctcct ctttatgtcg tctcaccaag
       61 aggtaaagca tatccactgt gctcagtatc ttgttatccg ctcacaatgt caatgttatc
      121 cgctcacatt tatatgcatc ctagaagcgg ccgcgaattc gagtcactaa gggctaacta
      181 actaattacg tagcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggcac
      241 gggcaaattg ctgaatattc cttttcttag acgtcaggtg gcaaacgcga agtaatcttt
      301 tcggttttaa agaaaaaggg cagggtggtg acaccttgcc cgtttttttg ccggagctag
      361 agctgtcaga ccaagtttac gagctcgctt ggactcctgt tgatagatcc agtaatgacc
      421 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      481 gagaatccaa gcactaggga cagtaagacg ggtaagcctg ttgatgatac cgctgcctta
      541 ctgggtgcat tagccagtct gaatgacctg tcacgggata atccgaagtg gtcagactgg
      601 aaaatcagag ggcaggaact gctgaacagc aaaaagtcag atagcaccac atagcagacc
      661 cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg gtagtttcct
      721 tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc ccattcactg ccagagccgt
      781 gagcgcagcg aactgaatgt cacgaa
//
