LOCUS       sequence-136465-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Wong AS, Choi GC, Cheng AA, Purcell O, Lu TK
  TITLE     Massively parallel high-order combinatorial genetics in human cells.
  JOURNAL   Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 
            2015 Aug 17.
  PUBMED    26280411
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3326"; 
            journalName: "Nat Biotechnol"; date: "2015-09"; volume: "33"; issue:
            "9"; pages: "952-61"
COMMENT     Sequence Label: pAWp7-10
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..502)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(521..568)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(616..812)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(624..834)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      630..765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(684..703)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
       61 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta
      121 gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
      181 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
      241 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
      301 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
      361 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
      421 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
      481 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga
      541 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca
      601 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      661 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
      721 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      781 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgg
//
