LOCUS       sequence-136471-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Lambda Red recombinases and a dominant negative MutL 
            allele all controlled by temperature sensitve cI857 repressor for 
            high precision and efficiency MAGE experiments. Ap resistance 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-2
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtcaaagaa ctagtgaaaa acagcctcga tgcaggtgcg acgcgtatcg atattgatat
       61 cgaacgcggt ggggcgaaac ttatccgcat tcgtgataac ggctgcggta tcaaaaaaga
      121 tgagctggcg ctggcgctgg ctcgtcatgc caccagtaaa atcgcctctc tggacgatct
      181 cgaagccatt atcagcctgg gctttcgcgg tgaggcgctg gcgagtatca gttcggtttc
      241 ccgcctgacg ctcacttcac gcaccgcaga acagcaggaa gcctggcagg cctatgccga
      301 agggcgcgat atgaacgtga cggtaaaacc ggcggcgcat cctgtgggga cgacgctgga
      361 ggtgctggat ctgttctaca acaccccggc gcggcgcaaa ttcctgcgca ccgagaaaac
      421 cgaatttaac cacattgatg agatcatccg ccgcattgcg ctggcgcgtt tcgacgtcac
      481 gatcaacctg tcgcataacg gtaaaattgt gcgtcagtac cgcgcagtgc cggaaggcgg
      541 gcaaaaagaa cggcgcttag gcgcgatttg cggcaccgct tttcttgaac aagcgctggc
      601 gattgaatgg caacacggcg atctcacgct acgcggctgg gtggccgatc caaatcacac
      661 cacgcccgca ctggcagaaa ttcagtattg ctacgtgaac ggtcgcatga tgcgcgatcg
      721 cctgatcaat cacgcgatcc gcca
//
