LOCUS       sequence-136477-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE1 with C-terminal NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE1
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             62..745
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGL"
ORIGIN
        1 cgtcagatcc gctagagatc cgcggccgct aatacgactc actataggga gagccgccac
       61 catgagctca gagactggcc cagtggctgt ggaccccaca ttgagacggc ggatcgagcc
      121 ccatgagttt gaggtattct tcgatccgag agagctccgc aaggagacct gcctgcttta
      181 cgaaattaat tgggggggcc ggcactccat ttggcgacat acatcacaga acactaacaa
      241 gcacgtcgaa gtcaacttca tcgagaagtt cacgacagaa agatatttct gtccgaacac
      301 aaggtgcagc attacctggt ttctcagctg gagcccatgc ggcgaatgta gtagggccat
      361 cactgaattc ctgtcaaggt atccccacgt cactctgttt atttacatcg caaggctgta
      421 ccaccacgct gacccccgca atcgacaagg cctgcgggat ttgatctctt caggtgtgac
      481 tatccaaatt atgactgagc aggagtcagg atactgctgg agaaactttg tgaattatag
      541 cccgagtaat gaagcccact ggcctaggta tccccatctg tgggtacgac tgtacgttct
      601 tgaactgtac tgcatcatac tgggcctgcc tccttgtctc aacattctga gaaggaagca
      661 gccacagctg acattcttta ccatcgctct tcagtcttgt cattaccagc gactgccccc
      721 acacattctc tgggccaccg ggttgaa
//
