LOCUS       sequence-136482-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL,
            thus lower level of expression of the enzyme..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagcgacctg cgtcggtagt caaagaacta gtgaaaaaca gcctcgatgc aggtgcgacg
       61 cgtatcgata ttgatatcga acgcggtggg gcgaaactta tccgcattcg tgataacggc
      121 tgcggtatca aaaaagatga gctggcgctg gcgctggctc gtcatgccac cagtaaaatc
      181 gcctctctgg acgatctcga agccattatc agcctgggct ttcgcggtga ggcgctggcg
      241 agtatcagtt cggtttcccg cctgacgctc acttcacgca ccgcagaaca gcaggaagcc
      301 tggcaggcct atgccgaagg gcgcgatatg aacgtgacgg taaaaccggc ggcgcatcct
      361 gtggggacga cgctggaggt gctggatctg ttctacaaca ccccggcgcg gcgcaaattc
      421 ctgcgcaccg agaaaaccga atttaaccac attgatgaga tcatccgccg cattgcgctg
      481 gcgcgtttcg acgtcacgat caacctgtcg cataacggta aaattgtgcg tcagtaccgc
      541 gcagtgccgg aaggcgggca aaaagaacgg cgcttaggcg cgatttgcgg caccgctttt
      601 cttgaacaag cgctggcgat tgaatggcaa cacggcgatc tcacgctacg cggctgggtg
      661 gccgatccaa atcacaccac gcccgcactg gcagaaattc agtattgcta cgtgaacggt
      721 cgcatgatgc gcgatcgcct gatcaatcac gcgatccgcc aggcctgcga agacaaactg
      781 ggggccgatc agcaaccggc atttgtgttg tatctggaga tcgacccaca tcaggtggac
      841 gtcaacgtgc accccgccaa acacgaagtg cgtttccatc agtcgcgtct ggtgcatgat
      901 t
//
