LOCUS       sequence-136483-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             64..750
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc
       61 accatgagct cagagactgg cccagtggct gtggacccca cattgagacg gcggatcgag
      121 ccccatgagt ttgaggtatt cttcgatccg agagagctcc gcaaggagac ctgcctgctt
      181 tacgaaatta attggggggg ccggcactcc atttggcgac atacatcaca gaacactaac
      241 aagcacgtcg aagtcaactt catcgagaag ttcacgacag aaagatattt ctgtccgaac
      301 acaaggtgca gcattacctg gtttctcagc tggagcccat gcggcgaatg tagtagggcc
      361 atcactgaat tcctgtcaag gtatccccac gtcactctgt ttatttacat cgcaaggctg
      421 taccaccacg ctgacccccg caatcgacaa ggcctgcggg atttgatctc ttcaggtgtg
      481 actatccaaa ttatgactga gcaggagtca ggatactgct ggagaaactt tgtgaattat
      541 agcccgagta atgaagccca ctggcctagg tatccccatc tgtgggtacg actgtacgtt
      601 cttgaactgt actgcatcat actgggcctg cctccttgtc tcaacattct gagaaggaag
      661 cagccacagc tgacattctt taccatcgct cttcagtctt gtcattacca gcgactgccc
      721 ccacacattc tctgggccac cgggttgaaa agcggcagcg agactcccgg gacctcagag
      781 tccgccacac ccgaa
//
