LOCUS       sequence-136486-         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL,
            thus lower level of expression of the enzyme..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-1
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             152..160
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    175..193
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 tgctttaagg cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct tttttgtgct
       61 catacgttaa atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt
      121 tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatggaac aacgagatgt
      181 gtataagaga cagctggcct gcccctccct tttggtgtcc aaccggctcg acgggggcag
      241 cgcaaggcgg tgcctccggc gggccactca atgcttgagt atactcacta gactttgctt
      301 cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac gggcttgctc tccgggcttc
      361 gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat atgtggacga tggccgcgag
      421 cggccaccgg ctggctcgct tcgctcggcc cgtggacaac cctgctggac aagctgatgg
      481 aca
//
