LOCUS       sequence-136490-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Lambda Red recombinases and a dominant negative MutL 
            allele all controlled by temperature sensitve cI857 repressor for 
            high precision and efficiency MAGE experiments. Kan resistance 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-3
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             154..162
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    177..195
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg
       61 ctcatacgtt aaatctatca ccgcaaggga taaatatcta acaccgtgcg tgttgactat
      121 tttacctctg gcggtgataa tggttgcatg tactaaggag gttgtatgga acaacgagat
      181 gtgtataaga gacagctggc ctgcccctcc cttttggtgt ccaaccggct cgacgggggc
      241 agcgcaaggc ggtgcctccg gcgggccact caatgcttga gtatactcac tagactttgc
      301 ttcgcaaagt cgtgaccgcc tacggcggct gcggcgccct acgggcttgc tctccgggct
      361 tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg atatgtggac gatggccgcg
      421 agcggccacc ggctggctcg cttcgctcgg cccgtggaca accctgctgg acaagctgat
      481 ggacaggctg cgcctgccca cgagcttgac cacagggatt gcccaccggc tacccagcct
      541 tcgaccacat acccaccggc tccaactgcg cggcctgcgg ccttgcccca tcaatttttt
      601 taa
//
