LOCUS       sequence-136496-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CelTag for the tagging and high affinity purification of 
            endogenous yeast proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Carrick BH, Hao L, Smaldino PJ, Engelke DR
  TITLE     A Novel Recombinant DNA System for High Efficiency Affinity 
            Purification of Proteins in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2015 Dec 29. pii: g3.115.025106. doi: 
            10.1534/g3.115.025106.
  PUBMED    26715090
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Dec 29. pii: g3.115.025106. doi: 
            10.1534/g3.115.025106."
COMMENT     Sequence Label: CelTag Plasmid
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(509..529)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(557..586)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(593..622)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(638..667)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(674..703)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(710..739)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(755..784)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(791..820)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(827..856)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtctattc tttaccccat acaagaacac
       61 cgttcaagta tgctgttacc tgatcccatt caacaaactg tgaagcagac ttgaatgagt
      121 agtcatttga ctgtgtatag ttactccagt cattctttgc aaatctacct tgtatctgaa
      181 catgtgcacc cggttcaaga gttccgcctg taaagcttat ttcaaggtag gtgtctgcgt
      241 tatttgttga ggaactcatt tttacaaatg ttccttttac atttgaagta attccgttgt
      301 agctgccgtt actgccgatt attgcagcat ggtcacacca gaaggtctga tctttctgtc
      361 cgtctactgt ataataatat ctcaatgtga gtttggacaa atcaattgca ctgcttccgg
      421 tattagtaac cttgaactga ggattgattg agttagtagt atctgaagga ttgctgttgt
      481 agaattcaac cttcaaattg cctgatacgc tttggaagta gaggttctcg tgattgatta
      541 atttttgttc accgttcaag tcttcctcgg agattagctt ttgttcaccg ttcaaatctt
      601 cttcagaaat caacttttgt tcaccgtcga gtccgttcaa gtcttcttct gagattaatt
      661 tttgttcacc gttcaagtct tcctcggaga ttagcttttg ttcaccgttc aaatcttctt
      721 cagaaatcaa cttttgttca ccgtcgagtc cgttcaagtc ttcttctgag attaattttt
      781 gttcaccgtt caagtcttcc tcggagatta gcttttgttc accgttcaaa tcttcttcag
      841 aaatcaactt ttgttcaccg tcgagtccgt tc
//
