LOCUS       sequence-136497-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator. This mutant is 
            deficient in circularisation. Under control of a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: pT7_circRAJ31_C873U
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      349..396
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      403..461
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(485..502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(736..755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttggggag tagggtcaag tgactcgaaa cgatagacaa cttgctttaa caagttggag
       61 atatagtctg ctctgcatgg tgacatgcag ctggatataa ttccggggta agattaacga
      121 ccttatctga acataatgct accgtttaat attcctaggg gatcaatatt aagctttacc
      181 gtcgtctcat ccgcaattaa gtccctatcc gctgtcactg tattggtctg ctagcatgtt
      241 ttcttgggtt aattgaggcc tgagtataag gtgacttata cttgtaatct atctaaacgg
      301 ggaacctctc tagtagacaa tcccgtgcta aattgtagga ctcaattgct agcataaccc
      361 cttggggcct ctaaacgggt cttgaggggt tttttgctgc agtccggcaa aaaaacggca
      421 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
      481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      661 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      781 ctgttccgac cctgccgctt accggatacc tgtccgcctt
//
