LOCUS       sequence-136498-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CelTag for the tagging and high affinity purification of 
            endogenous yeast proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Carrick BH, Hao L, Smaldino PJ, Engelke DR
  TITLE     A Novel Recombinant DNA System for High Efficiency Affinity 
            Purification of Proteins in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2015 Dec 29. pii: g3.115.025106. doi: 
            10.1534/g3.115.025106.
  PUBMED    26715090
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Dec 29. pii: g3.115.025106. doi: 
            10.1534/g3.115.025106."
COMMENT     Sequence Label: CelTag Plasmid
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..566
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     CDS             594..614
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gctcccggcc gccatggcgg ccgcgggaat tcgattaatc gatacatatg cccgggttaa
       61 ttaacggtga acaaaagcta atctccgagg aagacttgaa cggtgaacaa aaattaatct
      121 cagaagaaga cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa gaagatttga
      181 acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
      241 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
      301 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
      361 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
      421 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
      481 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
      541 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatcaat cacgagaacc
      601 tctacttcca aagcgtatca ggcaatttga aggttgaatt ctacaacagc aatccttcag
      661 atactactaa ctcaatcaat cctcagttca aggttactaa taccggaagc agtgcaattg
      721 atttgtccaa actcacattg agatattatt atacagtaga cggacagaaa gatcagacct
      781 tctggtgtga ccatgctgca ataatcggca gtaacggcag ctacaacgga attacttcaa
      841 atgtaaaa
//
