LOCUS       sequence-136501-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by 
            RAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: cisRAJ31-GFP-LAA
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    55..74
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    108..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             171..182
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg actcgaattc
       61 gcggccgctt ctaggatgca tataaatgtg agcggataac attgacattg tgagcggata
      121 acaagatact gagcacagtg gatatgcttt acctcttggt gagacgacat aaagaggaga
      181 aacataatat gactagccgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
      241 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
      301 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
      361 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
      421 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
      481 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
      541 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
      601 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
      661 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
      721 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
      781 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
      841 atcatatg
//
