LOCUS       sequence-136508-        3155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for N-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS234
FEATURES             Location/Qualifiers
     source          1..3155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(60..88)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    647..680
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2644..2655
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2804..2837
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3027..3047
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 taccgaagag cggatccatg ggctcttcga tgagcaaagg agaagaactt ttcactggag
       61 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      121 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      181 gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt
      241 taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc ttctcgagat
      301 acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt
      421 tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg
      481 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      541 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      601 aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaacataa cttcgtataa
      661 tgtatgctat acgaagttat ttctagacat tctctaatga aaaaatcttt cagttgaaat
      721 tgaaaatgag ttaaagttgg agtttttatt gaaaacagat ttccgtgtga ttagtgtttt
      781 tagcgagtgt gacaggacag cgaaaaaata tagaaacaag gggggaactg aaaagcttag
      841 gaatgcattg aacatgagaa ggggaagggg aaggaacaaa ctagacagga attattggaa
      901 tttaatcaca tttggagttt tttttctatt cgacagaata attatccaga acatttttgt
      961 attaaatatt tatgcatcat atgagtagtc ggctttgttg tgcatgacga gtttgttatc
     1021 gacgaaatag aagctgtcag aacgagtctc gtttggattg ttgatcatgt cgtccactga
     1081 aaaagagatt agtcttttga attgtacttt ttagaataat gactcactga gttgttgaga
     1141 gagttgaggg aactcataga tatgttcaca gttgtttcgt gaattcggaa tacagaatcc
     1201 gaattcaaag tcaaaacact tcagaaggcg atctttgaag aagtgacgtt cgatcattcg
     1261 gaaatgatgg attgggatgt ctcctacttt gaattccaca gttgctccga ccgttttgag
     1321 tttcaaaaag tttggagcaa atctataacg cacgtatctt gccgactcct gtggcgactc
     1381 atcattctcc tgatcgttct ccggtttggc gatctcgaaa agcacttgct cagtgtccag
     1441 atcacggatt tggaacttgg tgaactcgat gttatagatg ttcgcagatg gggagcataa
     1501 gaatcctaaa tttatgtttt aaactgaaat ccaaagggag caagatacct tgagtgattc
     1561 ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc tccgattccg
     1621 ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat ctggaaatat ggaaaagttc
     1681 aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag agttagttga gcggtttctc
     1741 tagttttctg agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa tcgattgttg
     1801 ttgctcggct ttcatatcgg tttggttgga agcggctgaa aacggaaaga agtggaagaa
     1861 ggaaaagagt gtggtgtgac aggaaaatgg taattagagg gtgccaaata accagctata
     1921 ttttgttttt ttttgaaaac atttttaaaa agaaaaatac gataatgata tcagatggat
     1981 ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt acatgtaatc aaaatacact
     2041 ttgtaaatta tcatttttat tgaaactcca ccatttttct atttataacg ctaataattt
     2101 gaaaaagaaa cctgttgcga accgcggggt gaatcccaaa aacgaatgcg ttttggtgga
     2161 gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg gaatagagag ctcactctta
     2221 accgagcagc acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt cttcttcttc
     2281 ttcttcgaat gattgacaga aatgggaaaa agaggaagat tgagaaggga aaaaggaagg
     2341 agaaaagaag cagaagaaga cgtcagagag gagaggaacg agcggaaaag cagcgggcgc
     2401 aagtcataga agtagcagag ctggggagaa gaagacacta tccaagaaag gaatgacgag
     2461 agagtatgca aaggggtata gggtgcagac agaataggaa cagaataaca gatgatgagc
     2521 caagaagagt tgaaaagggc gatgaatttg tcatgtaact taatttgggt caatttgagc
     2581 atgatgaatt gaaatcatcc cttgttggga gttaataacc ggtttgttat cagaaaccct
     2641 gtaatagaag ggcgccctaa ctttgagcca attcatcccg gtttctgtca aatatatcaa
     2701 aaagtggtca actgacaaat tgtttttgat attataataa acattttatc cgttaacaat
     2761 tttcgaatac tttttacaag gacttggata aattggctca aagataactt cgtataatgt
     2821 atgctatacg aagttattaa ctaatctgat ttaaattttc agaacttcaa aattagacac
     2881 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     2941 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     3001 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     3061 acacatggca tggatgaact atacaaaggt ggcacaggag gaacgggcgg tagtggaggc
     3121 actggtcgaa gagcggatcc atgggctctt cgcca
//
