LOCUS       sequence-136511-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutant FLAG-CARD14 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Berki DM, Liu L, Choon SE, David Burden A, Griffiths CE, Navarini 
            AA, Tan ES, Irvine AD, Ranki A, Ogo T, Petrof G, Mahil SK, Duckworth
            M, Allen MH, Vito P, Trembath RC, McGrath J, Smith CH, Capon F, 
            Barker JN
  TITLE     Activating CARD14 Mutations Are Associated With Generalized Pustular
            Psoriasis but Rarely Account for Familial Recurrence in Psoriasis 
            Vulgaris.
  JOURNAL   J Invest Dermatol. 2015 Jul 23. doi: 10.1038/jid.2015.288.
  PUBMED    26203641
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Invest 
            Dermatol. 2015 Jul 23. doi: 10.1038/jid.2015.288."
COMMENT     Sequence Label: pcDNA3-CARD14-E138A
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttaccatg
      121 gactacaagg acgacgatga caaggtacca ggatccagtg tggtggaatt catgggggaa
      181 ctgtgccgca gggactccgc actcacggca ctggacgagg agacactgtg ggagatgatg
      241 gagagccacc gccacaggat cgtacgctgc atctgcccca gccgcctcac cccctacctg
      301 cgccaggcca aggtgctgtg ccagctggac gaggaggagg tgctgcacag cccccggctc
      361 accaacagcg ccatgcgggc cgggcacttg ctggatttgc tgaagactcg agggaagaac
      421 ggggccatcg ccttcctgga gagcctgaag ttccacaacc ctgacgtcta caccctggtc
      481 accgggctgc agcctgatgt tgacttcagt aactttagcg gtctcatgga gacatccaag
      541 ctgaccgagt gcctggctgg ggccatcggc agcctgcagg aggcgctgaa ccaggaaaag
      601 gggcagaagg aggtgctgct gcggcggtgc cagcagctgc aggagcacct gggcctggcc
      661 gagacccgtg ccgagggcct gcaccagctg gaggctgacc acagccgcat gaagcgtgag
      721 gttagcgcac acttccatga ggtgctgagg ctgaaggacg agatgctcag cctctcgctg
      781 cactatagca atgcgctgca ggaaaaggag ctggccgcct cacgctgccg cagcctgcag
      841 gaggagctgt atctactgaa gcaggagctg cagcgagcca acatggtttc ctcctgtgag
      901 ctggaattgc aagagcagtc cctgaggaca gccagcgacc aggagtccgg ggatgaggag
      961 ctgaaccgcc tga
//
