LOCUS       sequence-136527-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for building 3n2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     855..874
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cgatcctcat cctgtctctt gatcagatca tgatcccctg cgccatcaga tccttggcgg
       61 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
      121 tggcaattcc gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgaa gacaaggcac gactctcaag ggcgcaataa
      241 aaaagccccc ggaaggtgat cttccggggg ctttctcatg cgttagttca ttaagctgct
      301 aaagcgtagt tttcgtcgtt tgctgcagac ccactttcac atttaagttg tttttctaat
      361 ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat
      421 aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct
      481 tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca
      541 gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat
      601 tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct
      661 ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct
      721 tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg
      781 gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct
      841 acacctagct tctgggcgag tttacgggtt gttaaacct
//
