LOCUS       sequence-136528-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The first of the two RNA lassos required for the RNA chain 
            construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Hassall J, MacDonald P, Cordero T, Rostain W, Jaramillo A
  TITLE     Design and Characterization of Topological Small RNAs.
  JOURNAL   Methods Mol Biol. 2015;1316:149-67. doi: 
            10.1007/978-1-4939-2730-2_13.
  PUBMED    25967060
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1316:149-67. doi"; pages: " 
            10.1007/978-1-4939-2730-2_13"
COMMENT     Sequence Label: Lasso 1
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     188..206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(210..253)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     271..290
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        271..289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      485..532
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      548..606
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(630..647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
       61 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      121 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
      181 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      241 taaggatgat ttctggaatt cgaagacgct taatacgact cactataggg aggctgtcct
      301 cgtccgtatg acgagagaag cctaccagag aaacacacga cgtaagtcgt ggtacattac
      361 ctggtacagt tctcttcaag ggacaaggct aacaacaact aacaacatac aatcaacata
      421 atttgccatc tgggtaacaa caacatacaa tcaacaatca accaaggctg tcctcgtcga
      481 tatcctagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgagcctctt
      541 cctgcagtcc ggcaaaaaaa cggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      601 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      721 gg
//
