LOCUS       sequence-136529-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for building 5n2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(465..484)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tcctcatcct gtctcttgat cagatcatga tcccctgcgc catcagatcc ttggcggcaa
       61 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
      121 caattccgac gtcggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
      181 gcccgctttc cagtcgggaa acctgaagac aaaaccgcat tgctgttcga ttcgttacca
      241 attgacagct agctcagtcc tactttagta tagagactga gcggtcggtc tatatactag
      301 agaagcgggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga
      361 aacatcttaa tcatgctggg gagggtttct aatgtctaga ttagataaaa gtaaagtgat
      421 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
      481 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
      541 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
      601 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
      661 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
      721 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
      781 attatatgca ctcagcgctg tgg
//
