LOCUS       sequence-136530-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split GFP assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Kodaka M, Yang Z, Nakagawa K, Maruyama J, Xu X, Sarkar A, Ichimura 
            A, Nasu Y, Ozawa T, Iwasa H, Ishigami-Yuasa M, Ito S, Kagechika H, 
            Hata Y
  TITLE     A new cell-based assay to evaluate myogenesis in mouse myoblast 
            C2C12 cells.
  JOURNAL   Exp Cell Res. 2015 Aug 15;336(2):171-81. doi: 
            10.1016/j.yexcr.2015.06.015. Epub 2015 Jun 24.
  PUBMED    26116467
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2015.06.015"; journalName: "Exp Cell Res"; date: 
            "2015-08-15- 15"; volume: "336"; issue: "2"; pages: "171-81"
COMMENT     Sequence Label: pQCXIP-GFP1-10
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(42..44,45..47,48..506)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITA"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcattggaac ggacctgcag gaattgatcc gcggccgatc catggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gcgcggcgag ggcgagggcg atgccaccat cggcaagctg accttgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      241 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctc cttcaaggac gacggcaaat
      361 acaagacccg cgccgtggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcaccga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca
      481 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      541 ccgtccgcca caacgtcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagaccg
      661 tcctgagcaa agaccc
//
