LOCUS       sequence-136555-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB41
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(135..163)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(503..536)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        540..587
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ttttcgaagc tcagaagatt gaatggcatg aatctggagg aggatctgag aacctttact
       61 tccaaggaga gaacctttac ttccaaggat ctggaggagg atctatgagt aaaggagaag
      121 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      181 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      241 ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa
      301 ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc
      361 aatgcttctc gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc
      421 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      481 gtaagtttaa acagttcggt acgaagttcc tatactttct agagaatagg aacttccctg
      541 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
      601 aaacccagga ggcagatcat gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca
      661 tttggctacc ctgccactca caccattcag gcgcctggcc gcgtgaattt gattggtgaa
      721 cacaccgact acaacgacgg tttcgttctg ccctgcgcga ttgattatca aaccgtgatc
      781 agttgtgcac cacgcgatga ccgtaaagtt cgcgtgatgg cagccgatta tgaaaatcag
      841 ctcgacgagt tttccctcga tgcgcccatt gtcgcacatg aaaactatca atgggctaac
      901 tacgttcgtg gcgtggtgaa acatctgcaa ctgcgtaaca acagcttcgg cggcgtggac
      961 atggtgatca gcg
//
