LOCUS       sequence-136557-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for C-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS235
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..499
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        527..602
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    610..846
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
ORIGIN
        1 tccaagaact cgtacaaaaa tgctctgaag taggtctcga gatcaatact gggaagacga
       61 aagtcttgcg aaaccgattc gctgacccca gtaaagtcta cttcggtagc ccttccccca
      121 ccacccagct cgacgacgtc gacgagtaca tctacctcgg tcgtcaaatc aacgcccaaa
      181 acaacttgat gccggaaatc caccgaagac gtcgagcagc ctgggctgca ttcaatggaa
      241 tcaagaatgc caccgactcc atcaccgaca agaagattcg tgcgaatctg ttcgactcaa
      301 ttgtccttcc agcgctcacc tacgtttcag aagcctggac attcaccaaa gctctatccg
      361 aacgagtacg aatcacacat gcctccccca caaattattg atctacatcc ccatttggta
      421 tagtgtctta caggactctc aatatgtcag ctgcatgata aatggtccct atataattga
      481 attgcaaatc taaatgtttg cgaagagcgg atccatgggc tcttcggttt tagagctaga
      541 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      601 gctttttttc aataatattc tagatatccc tttttgaaac taaaatgaaa tactctaaat
      661 ttttgagttc aatgtttcaa ctaatcttgg ttaaaggtaa attcccggag ggacgatttt
      721 aaagttgcca gagttgatta aattggtcca aaaaatttta ttaggaaact cgaaaaaaaa
      781 ttgatttttt ttgacaatat tccaaagaaa attacaaaat ctcgacgcca aacatagtcg
      841 gctgtg
//
