LOCUS       sequence-136563-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pMB72
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..83)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(423..456)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        460..507
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ggaggaggat ctggaggagg aggaatgagt aaaggagaag aacttttcac tggagttgtc
       61 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      121 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      181 ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
      241 tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca
      301 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt
      421 acgaagttcc tatactttct agagaatagg aacttccctg ttgacaatta atcatcggca
      481 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      541 gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca
      601 caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg
      661 tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agttgtgcac cacgcgatga
      721 ccgtaaagtt cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcga
      781 tgcgcccatt gtcgcacatg aaaactatca atgggctaac tacgttcgtg gcgtggtgaa
      841 acatctgcaa ctgcgtaaca acagcttcgg cggcgtggac atggtgatca gcggcaatgt
      901 gccgcagggt gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt
      961 gcagcagct
//
