LOCUS       sequence-136564-       12649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-17::2xNLS-FLP-D5 Expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12649)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 12649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS260
FEATURES             Location/Qualifiers
     source          1..12649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5881..5897
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5881..5897
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5917..5937
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    9163..9187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9196..9216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10585..10656
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(10660..10684)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11117..11137)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11145..11162)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11145..11161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgtac
     5941 accaatcatt tctcccctcc gaaatgtgta gtcgtcatga caaagtggtg acactggatt
     6001 acggtaggct ccgcccactt cttggaaaag tgacgtgggc tcggtgagcc ctaaagcttt
     6061 tggttttcac aattttctgg tttttgggat ttttgcggga attttgagag gagaaagttg
     6121 ggagagaaga tattggaatg gtccccgggg agctgtacat ttatacatct agactgaagc
     6181 tggggtattg caaaaagaga aaagcctatc actttctttc catcctgcat acttgccaaa
     6241 aagttttggc tgagcttatt tttaaaattg gaattttttg gaattttcag aaaagaaaaa
     6301 gttttttctt tttttttaat ttaaaaaatt tttgctattt attgcatggt actacagaaa
     6361 aacttgaatg aaataaaaaa tttggaaaat tcaagaaaac tttttttttc atagtttatt
     6421 tattcgagtt ttaatgtggc ttttattttg ttcggttttt gttttaaaaa gattttatac
     6481 aaaatttcaa gaatttacaa tttttccgtt gtaaaaaatc caaaaaaaaa tccaaaaaaa
     6541 tagttcagac ctttttttaa attttaaatt taaaaattca ataccaaaaa attaaaaaaa
     6601 attagttttt ccgagaataa aggaaaaatg ttgaaaaaat attatatata aaatttccag
     6661 aaaatgtgaa aactccaaaa aactgcagac ttttccccaa actagctatt ttttgaattc
     6721 attaccttta aaggtggagt accgaaattt aagactttgc ttttttagac ccaaaatggt
     6781 ccaaaactac caaatttcgt aatgagacgt tctgaaaatt tttcaaaaaa atgttatggg
     6841 agctcaaagt ttttgaaaaa atggtatatt tttagctaat tttaaccact tcttggcgtc
     6901 gcagcggttg gaactcaatt ttaattaaat tatcatcctt caatgtcctt caatctttgc
     6961 aatttcaatc gaccatacgc gttcagagca tttttttttt tcgtttttcc tcccgtccag
     7021 cacatattta aatattctct ctccccctcc cctggttcct aaaaacttgg gaaaggggaa
     7081 tttcgaagga ggtggaagct tcttcttctc cccgagcaca cacacacatc ctctcttcaa
     7141 aatctttaag aaatagtgcg tgtgaatgac tagacgctga agctaacttg gtttccgcga
     7201 acaatcccaa tcagtgcctg tgccgaggct tcggagaaga agagattgac gtcgtctttt
     7261 tctttttttg tgaaacagaa taaaaaagaa tacgaaatgg actttttcac acattttcgt
     7321 tacttttcca gaaaaaaacg aagctctagc catggaagtt tgaattgaaa acctaaaccc
     7381 cgatgtgtat tcatttgagc ttaagcctaa gcctaattct acgcctaagc ataagcctaa
     7441 gcctatgttc aagcctaagc caaattgtag aacgttacca aaagatcaaa cctatttttc
     7501 ctacagaaaa ggtctaatga taactgttaa gcgagagaca aggagagata tatatcaaaa
     7561 actagcccaa actgtgatta acatctatta actgacctca aaaaaaaaaa tagtagagat
     7621 taaatactgc tcaaaaagtt tcttggaaaa gtaaattgtt aggtttctgg ggcctgtaaa
     7681 actggaccga gttttgaaga atttttttaa cttttcaaag tttggcgtca ctgaaagctt
     7741 tatattgtac atgttccacc aattttcttt tgtatccgtt ttttctgtct caaaacggag
     7801 ctcgagccca tgacgtgtgg ataccgtgag aatccgccca attccttcac atcgatctca
     7861 tatttctttc ccagtttata ttgaattccc gttgaattgc ggctttgcag gggagaagat
     7921 gtgcgtcttt atattttttt ttctatcctt acccgccgtg aatccggctc gaattgagcc
     7981 aatttttatg aatagtagag ctaattgact tcgtctccct agaattgatg aggtaaagct
     8041 cgtggggaga tatggagatt tttcgatttt ttttccagta attttcagtt caaaatttga
     8101 gcttttgagc aggagttttg agatttgtgc caaaatttcc tattattttt tcaaatattt
     8161 ttgcttttgt aaaaaaggtg tagtagaagt tttgtagtgc cactattttt tttttgaaaa
     8221 ttatgttttg gcggaaagtt ctttaaaaaa gttaatcaaa atttttggcg ctttagtcaa
     8281 attaattttc tggcaaaaat attagtaatt ttggcgtgag ttcaattttc ccattcttaa
     8341 aatttcacac aattaagaat tttaagattt gggttttttt ttcaacaaaa aatcaaatat
     8401 ttacatgaaa attcggcggg aaataaaaaa aaatattccg attcaatgtg tgaataactt
     8461 tttgcgggga attcaaattt gcattattta aacttttttt aagctcgaaa aattgaaatt
     8521 cttaaataaa taaataaaat tttcctcgaa ttttgaatgt ttcgattttt tagcgttatt
     8581 ttttcaaaat ttccactaaa actctagttc taaagttcta aaactttaaa aacttcctaa
     8641 tgttgtggtc ttgtgttttt ttggcagcga tttccaaatt tctgaaattc caacaaaaaa
     8701 accactacgt cagtttgcat atccaacccc cccccccccc cccccccctc tgagccaatc
     8761 aatcatgtgc attttgaatt ggcgccaagg caatcttcgt aaaaaataac aatagagtca
     8821 tccctcagaa cagaaaagaa acccgcccgg gggctcaaat acacatgtca tttttctttt
     8881 ttcgcctgat ttttagccta aattagtgtt tagcaatttt ttaaggtaac tcggtggagt
     8941 catttgaact ttttaaacta aaatttcaga aaaaattctt tttttttgtc aaaaaataat
     9001 tttcccgccg gaatttttca aaaaaataat ttttatttta tttttcaagt tccttgtttc
     9061 ctctttattt ttttattttg cccccaaaag ttttacacta aaatatttaa ttttcaggta
     9121 attcatcgaa agtctttcta ttttccgcat ctcttgttca aaacaagttt gtacaaaaaa
     9181 gcaggctaaa aaatgccaaa gaagaagcgt aaggtcccac aatttgacat cctttgtaaa
     9241 accccaccaa aggtcctcgt ccgtcaattc gtcgagcgtt tcgagcgtcc atccggagag
     9301 aagatcgccc tctgcgccgc cgagctcacc tacctctgct ggatgatcac ccacaacgga
     9361 accgccatca agcgtgccac cttcatgtcc tacaacacca tcatctccaa ctccctctcc
     9421 ttcgacatcg tcaacaagtc cctccaattc aagtacaaga cccaaaaggc caccatcctc
     9481 gaggcctccc tcaagaagct catcccagcc tgggagttca ccatcatccc atactacgga
     9541 caaaagcacc aatccgacat caccgacatc gtctcctccc tccaactcca attcgagtcc
     9601 tccgaggagg ccgacaaggg aaactcccac tccaagaagg taagtttaaa catatatata
     9661 ctaactaacc ctgattattt aaattttcag atgctcaagg ccctcctctc cgagggagag
     9721 tccatctggg agatcaccga gaagatcctc aactccttcg agtacacctc ccgtttcacc
     9781 aagaccaaga ccctctacca attcctcttc ctcgccacct tcatcaactg cggacgtttc
     9841 tccgacatca agaacgtcga cccaaagtcc ttcaagctcg tccaaaacaa gtacctcgga
     9901 gtcatcatcc aatgcctcgt caccgagacc aagacctccg tctcccgtca catctacttc
     9961 ttctccgccc gtggacgtat cgacccactc gtctacctcg acgagttcct ccgtaactcc
    10021 gagccagtcc tcaagcgtgt caaccgtacc ggaaactcct cctccaacaa gcaagagtac
    10081 caactcctca aggacaacct cgtccgttcc tacaacaagg taagtttaaa cagttcggta
    10141 ctaactaacc atacatattt aaattttcag gccctcaaga agaacgcccc atactccatc
    10201 ttcgccatca agaacggacc aaagtcccac atcggacgtc acctcatgac ctccttcctc
    10261 tccatgaagg gactcaccga gctcaccaac gtcgtcggaa actggtccga caagcgtgcc
    10321 tccgccgtcg cccgtaccac ctacacccac caaatcaccg ccatcccaga ccactacttc
    10381 gccctcgtct cccgttacta cgcctacgac ccaatctcca aggagatgat cgccctcaag
    10441 gacgagacca acccaatcga ggagtggcaa cacatcgagc aactcaaggg ttccgccgag
    10501 ggatccatcc gttacccagc ctggaacgga atcatctccc aagaggtcct cgactacctc
    10561 tcctcctaca tcaaccgtcg tatctcccgt cgtcgtaagg ccaacccaac caagctctcc
    10621 gagaacgcca agaagctcgc caaggaggtc gagaactaaa cccagctttc ttgtacaaag
    10681 tggggatgat cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt
    10741 tttttcctat tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat
    10801 tcaatccaaa tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct
    10861 ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg
    10921 gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca
    10981 caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa
    11041 tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag
    11101 aattgtcctc gcttggcaac tttattatac atagttgata attcactggc cgtcgtttta
    11161 cactcgagac gtacggtgcg cgcgatgcat tcgaagatct gcccactagt gagtcgtatt
    11221 ataagtgcaa gtaagatcag tgtttgtttc gatatcagtc tgtttcgtaa cggtcttctg
    11281 tataactaca aaaaagaata aaaaaccgta tcacaactga gaaaaaagac aaaacttatt
    11341 gaacagtgtt cctttcaaaa aattattttg taaaaaatcg agtttttctc caaaataacg
    11401 cacgcaatca aaaacaagaa aactaacgtg ttctcctggg cgtggaactc cttatcaaga
    11461 ttaccattat tgtctgtcag aattgatatt tcaattattt catatctaga caaacccggg
    11521 tgtcttgcat ttattagtac tgtgagttta catacaaacg aggcttactc agataacata
    11581 gagtcacctc taattagcaa aaacccattt ctaattagct atttcgacca gttctaaagt
    11641 caccgtaatt agaaatggtt tttgtagctt ttctaattag aaatgacccc gagataagtg
    11701 aaatatggca ttttctgggt tttctagtaa gaaatgatcc cgcacgtagt gattccgcgt
    11761 tacaagccaa attcaaatta aaaaagtata ttacacaatt caagttacat tattttcagg
    11821 aaacaatggc gaagagcaaa gcagagattc aaaaggagga aagagacaac agatatgaac
    11881 gaagacttct tgaaaagaga aagagacatg aggtgagtaa ccatgataat aaataatcaa
    11941 gtaagggcta tagatttata aattctggag ttaacagttt cgaaaaaaag tagttttcat
    12001 atgtgcgaag gaatatcaaa aataggggaa tagcctaaac gagtgagaaa attaaatgtt
    12061 tgttattttt tcaaaaatgt ctgcagggcc gttatccttt caaatgcact ctatcaaaca
    12121 tagatcatat ccatcatatc ttatccagaa tttatatcat atccaagatt gagagcgttt
    12181 caaggaaaat ctcaaacaac catacaattt tgtagatcga caacaaggcc tccacttctt
    12241 catcgatggc ctctgcagca cctgcaccac caagtttgta tttttcatat taatataaag
    12301 tgagccacgc atagtcgttt ttaggatttt tgccagatcg atgtcgcgca aacagactgt
    12361 ttttttttct ttgaaagccg tgcgtgacat gggccacgga ttgtaacgcc gcgctggatg
    12421 gataaaaatt tattcagttt tttagccttt acaaaatatg gttcgactac tttctttatt
    12481 tttacatttt tataggtgct cattgagctg agatattttg ggtataatcg gaaaatcgct
    12541 gattttggtc atttttctgc actaaagatt acacaaaaag ttcagtattt agattcattg
    12601 ttgaaaattg gggatgatct acagatacat atcgccgtgt tcaaaaaca
//
