LOCUS       sequence-136567-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-47::2xNLS-FLP-D5 Expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS360
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(4..10,11..28,29..34))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    49..70
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(131..719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(211..230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      830..1339
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(917..936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1123..1144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(1381..2172,2173..2241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2004..2023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2242..2346)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2552..2574
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2567..2583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2587..2605
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4464..4475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    6541..6561
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7816..7840
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7849..7869
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9238..9309
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(9313..9337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9770..9790)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9798..9814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11516..11532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11516..11532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11529..11551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11540..11556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
       61 actcacatta catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      541 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      661 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta cgcgccctgt
      841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgctttc
      961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1021 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1141 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     1261 aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
     1321 tctgattatc aaccggggta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1981 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     2041 catcattgga gaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2341 tccgcgcaca tttccccgaa aagtgccacc tgacgtagtt aacaaaaaaa agcccgccga
     2401 agcgggcttt attaccaagc gaagcgccat tcgccattca ggctgcgcaa ctgttgggaa
     2461 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2521 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2581 agtccgtaat acgactcact ataggccttg actagagggt acccatagcc tgcaggattc
     2641 catatttgaa gcggcacaat atgcatcatt catactgttg atggatgcct gtgtggcggt
     2701 cctctattga tttttgctgt ttttatataa tacttcttaa attgttcaaa tgaactagct
     2761 ttgctaatgt aagtaataaa ttatcaattc atgctacaac cttattatct gacttttcag
     2821 gcagccatgt acattgtgat tgggactttt gggaattgat aaattactca attgagttca
     2881 aaaggtaatt acaacttttt ccacatgaaa agtaattttc cgatattcac aggttacatc
     2941 aagggctacc cggtcaacaa caacgagaag ttcctgtaca gatttgagtt ggagccaaat
     3001 gccatagtca atgggcccca aaactccaaa cacaccagtc acaaaagaca atgttactca
     3061 gtttagccca atatttatct atgttttttg ttttgttttt tatgcaaata aaatttctgt
     3121 tacactataa attgttataa aatcagaatc ttacttccga atcagttttg ggaacacaaa
     3181 gatagaaacg caaagaagat ccaactatca acaatgttta ctagaccggg gctcgccatg
     3241 tttggcccat taggtcagac aaaaatttct gatctttaaa ccttattgat taaaactaat
     3301 ttttgcccga cttttttgac acatgcacta gacgcttgta ctcaaaattt ggaaaccttg
     3361 ctcaaatcca ctactcagat atgacagtag catcgataat tgaaccatta tcctgaaatc
     3421 ccctttcctg tcccggttca ttttccaatt ttttcaacgg tggaagaagg ggcaaatttt
     3481 ataccaaaac gtctccgttt atgacccacc ccctatgcaa aataaacggc tctgggagaa
     3541 atagtacaga aaacgcggag cctgcttgat tccttctccg aggcgacaaa aaacgcatca
     3601 ttcgtattgt aagatgcatg tgtggctggc ctcttttgaa ttttactggt tttttatatt
     3661 tctactaata tttcgaaaaa ttttgtaagt aaatcattga ttacgtttac aaaaatttag
     3721 aaacatttca aaatgattac ttctaaatta attatattta caggttagtt cgagttactt
     3781 atgaccttcc aatccgccat atccgccaag gacgctccaa ttaatttttt agaattattt
     3841 tttaacacat gtattatttt ttaacaataa attattcatg aaatcatcaa atgtatatgt
     3901 tctactataa tatttaggtc aaactataga aaagaaatcc ttgtttttgg aacaaaggag
     3961 ttcagatcct gtgcatttga tcttggatcc acatctttgg tccatgggaa agcttggcac
     4021 tgatcaagaa ggattcttca aatatctaaa attgccggct gcccgtagaa ttccatcagt
     4081 tttccagagt tttagtgaaa atttgcatcc cgttagaaac aatctacaca acggtaaaaa
     4141 ggacccatta tttttaccgt accgttttcc tttatggccc tctatgcaaa aaattatagc
     4201 gtggaggggc agtacagaat aataaagtgt gagaaaaaaa actgctctgc gagaaatagt
     4261 acagcaaacg cggagcatgc ttgattctat aaatatggta gcaaactcac ttcgtctttg
     4321 agccaattta tccaagtcct tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt
     4381 attataatat caaaaacaat ttgtcagttg accacttttt gatatatttg acagaaaccg
     4441 ggatgaattg gctcaaagtt agggcgccct tctattacag ggtttctgat aacaaaccgg
     4501 ttattaactc ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag
     4561 ttacatgaca aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt
     4621 cctattctgt ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata
     4681 gtgtcttctt ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg
     4741 ttcctctcct ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat
     4801 cttcctcttt ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt
     4861 catttcattt ttttttctgt cggtgtgtgc tgctcggtta agagtgagct ctctattcca
     4921 cgtcttcttc tttttcttct tcgattcgaa tcaatcactc caccaaaacg cattcgtttt
     4981 tgggattcac cccgcggttc gcaacaggtt tctttttcaa attattagcg ttataaatag
     5041 aaaaatggtg gagtttcaat aaaaatgata atttacaaag tgtattttga ttacatgtac
     5101 tcaaaaaggt tgaaattttt cataccagtt ttccggaaat ccatctgata tcattatcgt
     5161 atttttcttt ttaaaaatgt tttcaaaaaa aaacaaaata tagctggtta tttggcaccc
     5221 tctaattacc attttcctgt cacaccacac tcttttcctt cttccacttc tttccgtttt
     5281 cagccgcttc caaccaaacc gatatgaaag ccgagcaaca acgatcgatt ccacccggct
     5341 cggcgacctt cccgtcgcag gtgagactca gaaaactaga gaaaccgctc aactaactct
     5401 tgatatccga tttcattctt ttctcttttc tttttgttga acttttccat atttccagat
     5461 gccacggcca ccaccaagca ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa
     5521 agctcaaatc actccgaacg acgttttagc acttccggga atcactcaag gtatcttgct
     5581 ccctttggat ttcagtttaa aacataaatt taggattctt atgctcccca tctgcgaaca
     5641 tctataacat cgagttcacc aagttccaaa tccgtgatct ggacactgag caagtgcttt
     5701 tcgagatcgc caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa
     5761 gatacgtgcg ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg
     5821 tggaattcaa agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct
     5881 tcaaagatcg ccttctgaag tgttttgact ttgaattcgg attctgtatt ccgaattcac
     5941 gaaacaactg tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat
     6001 tattctaaaa agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca
     6061 atccaaacga gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca
     6121 acaaagccga ctactcatat gatgcataaa tatttaatac aaaaatgttc tggataatta
     6181 ttctgtcgaa tagaaaaaaa actccaaatg tgattaaatt ccaataattc ctgtctagtt
     6241 tgttccttcc ccttcccctt ctcatgttca atgcattcct aagcttttca gttcccccct
     6301 tgtttctata ttttttcgct gtcctgtcac actcgctaaa aacactaatc acacggaaat
     6361 ctgttttcaa taaaaactcc aactttaact cattttcaat ttcaactgaa agattttttc
     6421 attagagaat gtctagaact aggcccgggc tacgtaatac gactcactta aggccttgac
     6481 tagagggtac cagagctcac ctaggcagga acagctatga ccatgattac gccaagctat
     6541 caactttgta tagaaaagtt gactaaactt ctacgtcaaa aagttgacaa aacaactttc
     6601 ttggaaaaaa agctgttggg atcccggaac agtcgaaagt cggtggcaag cgccgaactg
     6661 ctgacggtct aaccggggca caaatcaggg gtgagcggca aacgattttt ccggcaaatc
     6721 ggcaaatcgg caaattgcca atattgaaat acccggcaaa tcggtaaata gccggaattg
     6781 aaaatttccg gcaaactggt aaaccgcaaa ttgctgattt gccgaatttg ccgggaagac
     6841 ggcaattgcc aaacatattc ggcaaattgt ggttttgcac tttttggaaa tttcagaatt
     6901 tcaatcggca aattgtgcac atcctatgaa ttttcctaca tctattttga aaagtaagca
     6961 aattctatga aaatatctaa agaaaaatgg aaaaaatttt caaaaaggca cagttttaag
     7021 tgtttccgtc taataaaaaa atccccctaa acacttccgg caaattgatg ttcggcaaat
     7081 ggcaaatcgg aaacttgccg aaaattacag tttccggtaa atcggcaaac cggcaaactg
     7141 cctgaattga aaagttccgt caaatcggca aaccgacaac acccctggca caaatgatgg
     7201 acatactgag gcaatttgcc ggttttccaa ttgcaggaaa ttttcaattc cggcagtgtg
     7261 ccgatttgcc ggaaatttta attcaggcaa attgccgatt tcccgatttc ccgatttgcc
     7321 ggaaaaaatc gtttgccgcc cacccctggg tctgaacctt gattgttaca aaacattttt
     7381 agctctttgg agaaataaaa tgaatctcgt aaaatttaat tgacgaggac gatattagct
     7441 gtctctttag accaaattca gaaaaaaaga aagaatactt cccaaatttc cggtccctct
     7501 ctcgtttttt ttgccaataa actcactata gtcgctggtt cccccctatt cacatttatt
     7561 ctaccaatcc atcagtggaa ccagaaaaaa gaagagcctt tcggtttgga gagtagggtc
     7621 taataatccc ccgtgctctt caaatcattg tgccaacaca cagacacact ttatgtgtgc
     7681 tcacacacac acgctatttg aagagcgaag acgacgacga cgcattcaga gctcttttcc
     7741 acgaaatttg ctccatcttt ccacaatctg tctttcctgt gagacgacag cgtcacattt
     7801 atttcattac agctgacaag tttgtacaaa aaagcaggct aaaaaatgcc aaagaagaag
     7861 cgtaaggtcc cacaatttga catcctttgt aaaaccccac caaaggtcct cgtccgtcaa
     7921 ttcgtcgagc gtttcgagcg tccatccgga gagaagatcg ccctctgcgc cgccgagctc
     7981 acctacctct gctggatgat cacccacaac ggaaccgcca tcaagcgtgc caccttcatg
     8041 tcctacaaca ccatcatctc caactccctc tccttcgaca tcgtcaacaa gtccctccaa
     8101 ttcaagtaca agacccaaaa ggccaccatc ctcgaggcct ccctcaagaa gctcatccca
     8161 gcctgggagt tcaccatcat cccatactac ggacaaaagc accaatccga catcaccgac
     8221 atcgtctcct ccctccaact ccaattcgag tcctccgagg aggccgacaa gggaaactcc
     8281 cactccaaga aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     8341 cagatgctca aggccctcct ctccgaggga gagtccatct gggagatcac cgagaagatc
     8401 ctcaactcct tcgagtacac ctcccgtttc accaagacca agaccctcta ccaattcctc
     8461 ttcctcgcca ccttcatcaa ctgcggacgt ttctccgaca tcaagaacgt cgacccaaag
     8521 tccttcaagc tcgtccaaaa caagtacctc ggagtcatca tccaatgcct cgtcaccgag
     8581 accaagacct ccgtctcccg tcacatctac ttcttctccg cccgtggacg tatcgaccca
     8641 ctcgtctacc tcgacgagtt cctccgtaac tccgagccag tcctcaagcg tgtcaaccgt
     8701 accggaaact cctcctccaa caagcaagag taccaactcc tcaaggacaa cctcgtccgt
     8761 tcctacaaca aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     8821 caggccctca agaagaacgc cccatactcc atcttcgcca tcaagaacgg accaaagtcc
     8881 cacatcggac gtcacctcat gacctccttc ctctccatga agggactcac cgagctcacc
     8941 aacgtcgtcg gaaactggtc cgacaagcgt gcctccgccg tcgcccgtac cacctacacc
     9001 caccaaatca ccgccatccc agaccactac ttcgccctcg tctcccgtta ctacgcctac
     9061 gacccaatct ccaaggagat gatcgccctc aaggacgaga ccaacccaat cgaggagtgg
     9121 caacacatcg agcaactcaa gggttccgcc gagggatcca tccgttaccc agcctggaac
     9181 ggaatcatct cccaagaggt cctcgactac ctctcctcct acatcaaccg tcgtatctcc
     9241 cgtcgtcgta aggccaaccc aaccaagctc tccgagaacg ccaagaagct cgccaaggag
     9301 gtcgagaact aaacccagct ttcttgtaca aagtggggat gatcgacgcc aacgtcgttg
     9361 aattttcaaa ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt
     9421 tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac attttttttt
     9481 ctctttccgt ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta
     9541 tttatcttct cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac
     9601 acaaaaggat gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa
     9661 atatggagga aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta
     9721 aaaaataagc aaagaaatta ggcgaacgat gagaattgtc ctcgcttggc aactttatta
     9781 tacatagttg ataattcact ggccgtcgtt ttacactcga gacgtacggt gcgcgcgatg
     9841 cattcgaaga tctgcccact agtgagtcgt attataagtg caagtaagat cagtgtttgt
     9901 atttaattca aaaacgcaga actcgtactg ttggatgcct gtgtagcggt cctctattga
     9961 tttttgctat tttttgataa ttttactgta aattatgagt tattccaatt aacattccta
    10021 gctaaatgta agttagcgac caatttttta gcaaccccat tttatgactt ttcagaatat
    10081 cgcctactgg gtcaacgaca cttgtttgga actaagggtc ttcccaattt tttttaaatc
    10141 ttcataggct tgacagaatc agggttcttc aagcagcttt gagagggaat caattgcaac
    10201 atttgatgtt aaatttatat tttttatagt tctctggtga aatggggacc accgcaaagc
    10261 cccttttatg tttttaactt gaataaaatc ttgtattaaa ttgtgattta tagatctgta
    10321 tgggtaattt gaaggaaaag aaagggagct cccaaaagtg ccttacagat ccaggcccaa
    10381 taaatgtaaa ctattgggca gtagtatggc aaaaatcgtg cggaatttga aaaacctaga
    10441 attaaaataa atattcaaca caaatgttcg agatatttta agttcggagt gtatatctag
    10501 aacatattgt acaagtaggt ctacgggatc gtctacggga aaatttgatt tcctcgaaga
    10561 tgtatttaat gaattctcta atgttgcagg tctttgtatt tgacagaatg ttggtttttg
    10621 aaacttattc gccataattg cttggctact acatgtctca tgccattgac actatcctgt
    10681 actcgaaatt tgttaagtaa ctacccaccg tccacttatg acgagagggt caattaaggt
    10741 cactccacaa aagtaaattt gaaaacacat gagaaaaagt gatctgaatt cccttgaagt
    10801 ggctaaaaca aagcagatga gggatacgac gtatgcgact actgagagat aaggtcgaaa
    10861 cttgataagg atgctgctta cctgcttagc aagtttcttc gcacaggaga aagtgacaag
    10921 ctaaactatc aaattcatct aaaacctcaa aaaattattt tggatgaaat aatgttttat
    10981 tttgagcaac gcataaggca aagaataaat taaaggcgat ccgcttagcg gcgagttccg
    11041 tcctcgaaga agattcctcc atcgatggcg cagtactttg gacagattcc acgctctcct
    11101 ggggttcctg gtggtccgac tggtcctggc tgtcctgggt ttccgtcggc tcctggcttg
    11161 ccgtctggtc ctggtggtcc ctttggtcca ggttgtcctg aaagatgtgg tgattagata
    11221 tggattgaaa aactatgaaa tgattacctg gggttccgtc gtttcctggt tgtccggatg
    11281 gtcctggtgg tccgactggt cctggctctc cggtggatcc tggctctccc ttttggactg
    11341 gctcgcagac agctggaagt ccctcgtctc ctggtgctcc tggtgctcct ggctttcctg
    11401 gtgctccgtt tggtcccttt ggtccttgct ctcctgctcg agacgtacgg tgcgcgcgat
    11461 gcattcgaag atctgcccac tagtgagtcg tattacgtag cttggcgtaa tcatggtcat
    11521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
//
