LOCUS       sequence-136585-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans germline CRE expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS328
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(151..170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(835..1344)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1030..1051)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1238..1257
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1455..2043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1944..1963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2104..2125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2140..2145,2146..2163,2164..2170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2178..2194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2183..2205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2202..2218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2202..2218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3808..3819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5885..5905
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5906..6505
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    6514..6538
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6550..6570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6571..7596
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     protein_bind    complement(7685..7709)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7714..8037
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(8041..8061)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8069..8085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9604..9620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9613..9635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        9841..9945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9946..10013,1,2..793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
       61 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      121 ttctccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
      181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaccccg
      841 gttgataatc agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta
      901 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      961 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg
     1021 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1081 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt
     1141 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     1201 agagcttgac ggggaaagcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1261 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1321 ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc
     1381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2101 atgtaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     2161 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     2221 tgggtcaccc aaggcgacaa cgtaagtgac tgaaggcatt aaaggtacat gcgtccgcgc
     2281 aagtgtccgc ttttgctggc ctaacaagta agagaaacaa gtaagaaaga gtaagagaaa
     2341 actcacccga ggtgtctctt ctagcaaccg cctattcaga taattttaag ccagttcgag
     2401 agttatttgt cgcatggtat agaagctcgt tatttacatt aaatttttag tcttcccgca
     2461 gaagtggacg atgcgatggg ctacttcaaa aatctgacgc cacgtgtcaa ggaccttcac
     2521 aagagcatga caaacttgga agatattagc aagtggcaga agaaggccag tttctctggc
     2581 acccttgaga attactcgcg tctcggtgac aagatcaatg tgaaaccatt tatggatgct
     2641 gttgatgcta gaatgggtgc cgaagctgat gccgtgaaag gggtataaag ctactgttgc
     2701 acgacgactc acagactaat cattccaggt cctcgcgatt tgtgaaaaat acaagtcatt
     2761 ctaccaaaac gagggaaaac ttcacgcgga cagtatcgcc aatttgaata ggactcggct
     2821 cgacatggac agtgcggcgg ataaatatgc gaacaacgag actgaagtta acaagactcg
     2881 tttggataac agtaccacgg aatttgaagt ggcttgtgag aggtttgtag gtctttcgta
     2941 ggcacgatat ccttatttga atgtgtttca gaatgcgaga actggcgaac ggaatcaaga
     3001 caattgaatc gaaccattct tcctggcaag acggtcttat gaaggagatt aaagtggcgt
     3061 tgcgtaaata aagaaaggat cctctttgaa aataaagatt ttctacattc acttattttt
     3121 ttagctttat acttttcaga tcgaatgaac tgtttccaac gtttccatat taattttttt
     3181 tagttaaaaa agttgggtat taacagtttt gttaacagtt ttgtcttccg acagatgtga
     3241 gacggaaaaa tggccaaaat tcgagatttt gactaaaatc agtgtagctt tttcaaaatt
     3301 ttgaaccggc atgaaaaagt ttgaaaagtt ttcgagaagt ttcaatccgt tcatttgagc
     3361 ccatgttggg tctataagat caaaatcgcc cgaactgtta gtcctccttt aagttacgat
     3421 tattaacaaa atgtcgccta agtttagttt cacacttgtc tcacactagt cgatgaactg
     3481 tgtgactact gtagtgagta cagtagggcc cgtgtaaaaa tacaaaaacc aagaaaaagt
     3541 aaaccaaaat aaaaaacgaa tttattgttc actacaacca aactgcaaag ttcttttctt
     3601 acccatcagc tcaatcgtgt acttgcgtgg aaacaaatat ggtagcaaac tcacttcgtc
     3661 tttgagccaa tttatccaag tccttgtaaa aagtattcga aaattgttaa cggataaaat
     3721 gtttattata atatcaaaaa caatttgtca gttgaccact ttttgatata tttgacagaa
     3781 accgggatga attggctcaa agttagggcg cccttctatt acagggtttc tgataacaaa
     3841 ccggttatta actcccaaca agggatgatt tcaattcatc atgctcaaat tgacccaaat
     3901 taagttacat gacaaattca tcgccctttt caactcttct tggctcatca tctgttattc
     3961 tgttcctatt ctgtctgcac cctatacccc tttgcatact ctctcgtcat tcctttcttg
     4021 gatagtgtct tcttctcccc agctctgcta cttctatgac ttgcgcccgc tgcttttccg
     4081 ctcgttcctc tcctctctga cgtcttcttc tgcttctttt ctccttcctt tttcccttct
     4141 caatcttcct ctttttccca tttctgtcaa tcattcgaag aagaagaaga agaagaccct
     4201 cattcatttc attttttttt ctgtcggtgt gtgctgctcg gttaagagtg agctctctat
     4261 tccacgtctt cttctttttc ttcttcgatt cgaatcaatc actccaccaa aacgcattcg
     4321 tttttgggat tcaccccgcg gttcgcaata ggtttctttt tcaaattatt agcgttataa
     4381 atagaaaaat ggtggagttt caataaaaat gataatttac aaagtgtatt ttgattacat
     4441 gtactcaaaa aggttgaaat ttttcatacc agttttccgg aaatccatct gatatcatta
     4501 tcgtattttt ctttttaaaa atgttttcaa aaaaaaacaa aatatagctg gttatttggc
     4561 accctctaat taccattttc ctgtcacacc acactctttt ccttcttcca cttctttccg
     4621 ttttcagccg cttccaacca aaccgatatg aaagccgagc aacaacgatc gattccaccc
     4681 ggctcggcga ccttcccgtc gcaggtgaga ctcagaaaac tagagaaacc gctcaactaa
     4741 ctcttgatat ccgatttcat tcttttctct tttctttttg ttgaactttt ccatatttcc
     4801 agatgccacg gccaccacca agcaccgaac aaggaatcac aacggaatcg gagcttgcga
     4861 agaaagctca aatcactccg aacgacgttt tagcacttcc gggaatcact caaggtatct
     4921 tgctcccttt ggatttcagt ttaaaacata aatttaggat tcttatgctc cccatctgcg
     4981 aacatctata acatcgagtt caccaagttc caaatccgtg atctggacac tgagcaagtg
     5041 cttttcgaga tcgccaaacc ggagaacgat caggagaatg atgagtcgcc acaggagtcg
     5101 gcaagatacg tgcgttatag atttgctcca aactttttga aactcaaaac ggtcggagca
     5161 actgtggaat tcaaagtagg agacatccca atccatcatt tccgaatgat cgaacgtcac
     5221 ttcttcaaag atcgccttct gaagtgtttt gactttgaat tcggattctg tattccgaat
     5281 tcacgaaaca actgtgaaca tatctatgag ttccctcaac tctctcaaca actcagtgag
     5341 tcattattct aaaaagtaca attcaaaaga ctaatctctt tttcagtgga cgacatgatc
     5401 aacaatccaa acgagactcg ttctgacagc ttctatttcg tcgataacaa actcgtcatg
     5461 cacaacaaag ccgactactc atatgatgca taaatattta atacaaaaat gttctggata
     5521 attattctgt cgaatagaaa aaaaactcca aatgtgatta aattccaata attcctgtct
     5581 agtttgttcc ttccccttcc ccttctcatg ttcaatgcat tcctaagctt ttcagttccc
     5641 cccttgtttc tatatttttt cgctgtcctg tcacactcgc taaaaacact aatcacacgg
     5701 aaatctgttt tcaataaaaa ctccaacttt aactcatttt caatttcaac tgaaagattt
     5761 tttcattaga gaatgtctag aactaggccc gggctacgta atacgactca cttaaggcct
     5821 tgactagagg gtaccagagc tcacctaggc aggaacagct atgaccatga ttacgccaag
     5881 ctatcaactt tgtatagaaa agttggcacc tttggtcttt tattgtcaac ttccattggt
     5941 tcttccattg tttctgttaa attaatgaat ttttcataaa ataaagacat tatacaatat
     6001 aaaaatgaag aatttattga aaataaactg ccagagagaa aaagtatgca acactcccgc
     6061 cgagagtgtt tgaaatggtg tacggtacat tttcgtgcta ggagttagat gtgcaggcag
     6121 caacgagagg gggagagatt tttttgggcc ttgtgaaatt aacgtgagtt ttctggtcat
     6181 ctgactaatc atgttggttt tttgttggtt tattttgttt ttatctttgt ttttatccag
     6241 attaggaaat ttaaatttta tgaatttata atgaggtcaa acattcagtc ccagcgtttt
     6301 tcctgttctc actgtttagt cgaattttta ttttaggctt tcaacaaatg ttctaactgt
     6361 cttatttgtg acctcacttt ttatattttt ttaattttta aaaatattag aagtttctag
     6421 gataattttt tcgactttta ttctctctac cgtccgcact cttcttactt ttaaattaaa
     6481 ttgttttttt ttcagttggg aaacactttg ctcacaagtt tgtacaaaaa agcaggctcc
     6541 atgggcgcac ccaagaagaa gaggaaggtg tccaatttac tgaccgtaca ccaaaatttg
     6601 cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc
     6661 agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg
     6721 tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt
     6781 cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat
     6841 ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat
     6901 gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca
     6961 aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat
     7021 agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg
     7081 ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg
     7141 agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag
     7201 gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct
     7261 gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct
     7321 gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg
     7381 atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc
     7441 cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa
     7501 gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa
     7561 acaggggcaa tggtgcgcct gctggaagat ggcgatggga gctctatgag ccgtagacga
     7621 aaagcgaatc cgacaaaact gagtgaaaac gcgaagaagc ttgccaagga agttgaaaat
     7681 tagtacccag ctttcttgta caaagtggga taaatgcaaa atcctttcaa gcattccctt
     7741 cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt atttgctttt
     7801 ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct gagtgaagtg
     7861 aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc aattccattt
     7921 tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat ttgtattgtg
     7981 ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa aaaagtctca
     8041 caactttatt atacatagtt gataattcac tggccgtcgt tttacactcg agacgtacgg
     8101 tgcgcgcgat gcattcgaag atctgcccac tagtgagtcg tattataagt gcaagtaaga
     8161 tcagtgtttg tatttaagac acacggccaa atttcttatt ttcctgctat tttctccaaa
     8221 tatgattaaa atttgttcat ttcaaaattg cctccaactt gttttggggc aaaaatttca
     8281 caaaacttca cagcaaaatg tgaaacaata ttgctatcta ttgaaaacat taatgatagt
     8341 cgagtgactt cttgaaccaa tactcatcgc atcaaatgcc ttttctcgaa tactctgtaa
     8401 taattggcat ataaaaacat acatactcat cagtttgaaa gctaaccttt accataatca
     8461 ttactaaaat tggatttaaa gccggacacc aagaaaaaag atacggtaga taaactgaca
     8521 gatgagaagt ctcgatacga aataatggca ttagaaaaga cacatagaat ggaatgtgga
     8581 tagtgagaaa tggtagaatg aactgaaaaa ttgagaattt aaggagaaat gttattttta
     8641 agctgctttg cttgaaactt tgaatttaaa attttagaac aattactgtt tcaaaatatt
     8701 tttattttta tttactccat ttgaaaaaaa agcaacattg cttgttcaat ttctgttatt
     8761 gccttacctg aaccataagt accatattca attgttgttg agacttcatc gaatttgtac
     8821 tccgacttcc cttatgaatt tcgtatctga taattgtcca acaaaccatc gcaattactg
     8881 tagttatcac tagaacagcc attatataaa tcgtacatgc ccatgaaatt gggtcagcaa
     8941 caaagtcatt caacgtgtaa tcatatcttt ctagtgatgc aattaaatat gcagattttc
     9001 cgattccatt cagattgtat acgatatatt ctgatagttg atcgtcagca gcatctggga
     9061 catagccaaa ggtgattaga actgtggata ttagagagac aataaatgat atgattgtgg
     9121 ttgtaaggac cacgtttttt tgtgttctcg ttgttctgaa agtataattt gtagattctt
     9181 aaactcagaa tttatcatat ttgaactccg ctgtttttga attaagtaat agctgaaaca
     9241 gtgaacatta gtttaaaaat ataattttac aggcacgcac aaacttcggt gtgggtacgt
     9301 attacctgcc tcctattgaa tattcattgt ttaaacgttt tcagaaaaaa agtgtacatg
     9361 ttttttggag tatgatgatg atgtagtcgc aaggcagata tgaaatgtag gtaacacagt
     9421 gcacagatgc gttttttgcc aacatcaagg gttgtaagta acttttcaga gaaaaacata
     9481 tagaaatttt gtttcaagta tcgtatcagt ttttttctga ttaacttata aaaagaactc
     9541 ttcgacaatt tgaatgctta atttaaaaaa attaagtcga atatttcagc atgccaatga
     9601 tcaactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcttcgctt ggtaataaag
     9781 cccgcttcgg cgggcttttt tttgttaact acgtcaggtg gcacttttcg gggaaatgtg
     9841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgc
//
