LOCUS       sequence-136591-        3077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap GFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS252
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..67)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    626..659
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2623..2634
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2783..2816
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3006..3026
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctcttcgat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
       61 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      121 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
      181 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
      241 tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc
      301 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      361 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
      421 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      481 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      541 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
      601 taagtttaaa catgatttta ctaacataac ttcgtataat gtatgctata cgaagttatt
      661 tctagacatt ctctaatgaa aaaatctttc agttgaaatt gaaaatgagt taaagttgga
      721 gtttttattg aaaacagatt tccgtgtgat tagtgttttt agcgagtgtg acaggacagc
      781 gaaaaaatat agaaacaagg ggggaactga aaagcttagg aatgcattga acatgagaag
      841 gggaagggga aggaacaaac tagacaggaa ttattggaat ttaatcacat ttggagtttt
      901 ttttctattc gacagaataa ttatccagaa catttttgta ttaaatattt atgcatcata
      961 tgagtagtcg gctttgttgt gcatgacgag tttgttatcg acgaaataga agctgtcaga
     1021 acgagtctcg tttggattgt tgatcatgtc gtccactgaa aaagagatta gtcttttgaa
     1081 ttgtactttt tagaataatg actcactgag ttgttgagag agttgaggga actcatagat
     1141 atgttcacag ttgtttcgtg aattcggaat acagaatccg aattcaaagt caaaacactt
     1201 cagaaggcga tctttgaaga agtgacgttc gatcattcgg aaatgatgga ttgggatgtc
     1261 tcctactttg aattccacag ttgctccgac cgttttgagt ttcaaaaagt ttggagcaaa
     1321 tctataacgc acgtatcttg ccgactcctg tggcgactca tcattctcct gatcgttctc
     1381 cggtttggcg atctcgaaaa gcacttgctc agtgtccaga tcacggattt ggaacttggt
     1441 gaactcgatg ttatagatgt tcgcagatgg ggagcataag aatcctaaat ttatgtttta
     1501 aactgaaatc caaagggagc aagatacctt gagtgattcc cggaagtgct aaaacgtcgt
     1561 tcggagtgat ttgagctttc ttcgcaagct ccgattccgt tgtgattcct tgttcggtgc
     1621 ttggtggtgg ccgtggcatc tggaaatatg gaaaagttca acaaaaagaa aagagaaaag
     1681 aatgaaatcg gatatcaaga gttagttgag cggtttctct agttttctga gtctcacctg
     1741 cgacgggaag gtcgccgagc cgggtggaat cgattgttgt tgctcggctt tcatatcggt
     1801 ttggttggaa gcggctgaaa acggaaagaa gtggaagaag gaaaagagtg tggtgtgaca
     1861 ggaaaatggt aattagaggg tgccaaataa ccagctatat tttgtttttt tttgaaaaca
     1921 tttttaaaaa gaaaaatacg ataatgatat cagatggatt tccggaaaac tggtatgaaa
     1981 aatttcaacc tttttgagta catgtaatca aaatacactt tgtaaattat catttttatt
     2041 gaaactccac catttttcta tttataacgc taataatttg aaaaagaaac ctgttgcgaa
     2101 ccgcggggtg aatcccaaaa acgaatgcgt tttggtggag tgattgattc gaatcgaaga
     2161 agaaaaagaa gaagacgtgg aatagagagc tcactcttaa ccgagcagca cacaccgaca
     2221 gaaaaaaaaa tgaaatgaat gagggtcttc ttcttcttct tcttcgaatg attgacagaa
     2281 atgggaaaaa gaggaagatt gagaagggaa aaaggaagga gaaaagaagc agaagaagac
     2341 gtcagagagg agaggaacga gcggaaaagc agcgggcgca agtcatagaa gtagcagagc
     2401 tggggagaag aagacactat ccaagaaagg aatgacgaga gagtatgcaa aggggtatag
     2461 ggtgcagaca gaataggaac agaataacag atgatgagcc aagaagagtt gaaaagggcg
     2521 atgaatttgt catgtaactt aatttgggtc aatttgagca tgatgaattg aaatcatccc
     2581 ttgttgggag ttaataaccg gtttgttatc agaaaccctg taatagaagg gcgccctaac
     2641 tttgagccaa ttcatcccgg tttctgtcaa atatatcaaa aagtggtcaa ctgacaaatt
     2701 gtttttgata ttataataaa cattttatcc gttaacaatt ttcgaatact ttttacaagg
     2761 acttggataa attggctcaa agataacttc gtataatgta tgctatacga agttattaac
     2821 taatctgatt taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt
     2881 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2941 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     3001 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     3061 tacaaaggtc gaagagc
//
