LOCUS       sequence-136593-        3155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Halotag + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS254
FEATURES             Location/Qualifiers
     source          1..3155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    549..582
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2546..2557
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2706..2739
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctcttcgat gatggctgaa attggtactg gctttccttt tgatccccac tacgttgagg
       61 ttcttggaga gcgcatgcac tacgttgatg ttggaccacg cgatggaacc ccagtccttt
      121 tccttcacgg aaacccaact tcttcttacg tttggcgcaa catcatccca catgttgctc
      181 caacccaccg ctgcatcgct ccagatctta tcggaatggg aaagtctgat aagccagatc
      241 ttggatactt cttcgatgat cacgtccgtt tcatggatgc tttcatcgag gcccttggac
      301 ttgaggaggt tgtccttgtt atccacgatt ggggatccgc tcttggattc cactgggcta
      361 agcgcaaccc agagcgcgtt aagggaatcg ctttcatgga attcatccgc ccaatcccaa
      421 cttgggatga atggccagag ttcgctcgcg agaccttcca agctttccgc accaccgatg
      481 ttggacgtaa gcttatcatc gatcaaaacg ttttcatcga aggtaagttt aaacatgatt
      541 ttactaacat aacttcgtat aatgtatgct atacgaagtt atttctagac attctctaat
      601 gaaaaaatct ttcagttgaa attgaaaatg agttaaagtt ggagttttta ttgaaaacag
      661 atttccgtgt gattagtgtt tttagcgagt gtgacaggac agcgaaaaaa tatagaaaca
      721 aggggggaac tgaaaagctt aggaatgcat tgaacatgag aaggggaagg ggaaggaaca
      781 aactagacag gaattattgg aatttaatca catttggagt tttttttcta ttcgacagaa
      841 taattatcca gaacattttt gtattaaata tttatgcatc atatgagtag tcggctttgt
      901 tgtgcatgac gagtttgtta tcgacgaaat agaagctgtc agaacgagtc tcgtttggat
      961 tgttgatcat gtcgtccact gaaaaagaga ttagtctttt gaattgtact ttttagaata
     1021 atgactcact gagttgttga gagagttgag ggaactcata gatatgttca cagttgtttc
     1081 gtgaattcgg aatacagaat ccgaattcaa agtcaaaaca cttcagaagg cgatctttga
     1141 agaagtgacg ttcgatcatt cggaaatgat ggattgggat gtctcctact ttgaattcca
     1201 cagttgctcc gaccgttttg agtttcaaaa agtttggagc aaatctataa cgcacgtatc
     1261 ttgccgactc ctgtggcgac tcatcattct cctgatcgtt ctccggtttg gcgatctcga
     1321 aaagcacttg ctcagtgtcc agatcacgga tttggaactt ggtgaactcg atgttataga
     1381 tgttcgcaga tggggagcat aagaatccta aatttatgtt ttaaactgaa atccaaaggg
     1441 agcaagatac cttgagtgat tcccggaagt gctaaaacgt cgttcggagt gatttgagct
     1501 ttcttcgcaa gctccgattc cgttgtgatt ccttgttcgg tgcttggtgg tggccgtggc
     1561 atctggaaat atggaaaagt tcaacaaaaa gaaaagagaa aagaatgaaa tcggatatca
     1621 agagttagtt gagcggtttc tctagttttc tgagtctcac ctgcgacggg aaggtcgccg
     1681 agccgggtgg aatcgattgt tgttgctcgg ctttcatatc ggtttggttg gaagcggctg
     1741 aaaacggaaa gaagtggaag aaggaaaaga gtgtggtgtg acaggaaaat ggtaattaga
     1801 gggtgccaaa taaccagcta tattttgttt ttttttgaaa acatttttaa aaagaaaaat
     1861 acgataatga tatcagatgg atttccggaa aactggtatg aaaaatttca acctttttga
     1921 gtacatgtaa tcaaaataca ctttgtaaat tatcattttt attgaaactc caccattttt
     1981 ctatttataa cgctaataat ttgaaaaaga aacctgttgc gaaccgcggg gtgaatccca
     2041 aaaacgaatg cgttttggtg gagtgattga ttcgaatcga agaagaaaaa gaagaagacg
     2101 tggaatagag agctcactct taaccgagca gcacacaccg acagaaaaaa aaatgaaatg
     2161 aatgagggtc ttcttcttct tcttcttcga atgattgaca gaaatgggaa aaagaggaag
     2221 attgagaagg gaaaaaggaa ggagaaaaga agcagaagaa gacgtcagag aggagaggaa
     2281 cgagcggaaa agcagcgggc gcaagtcata gaagtagcag agctggggag aagaagacac
     2341 tatccaagaa aggaatgacg agagagtatg caaaggggta tagggtgcag acagaatagg
     2401 aacagaataa cagatgatga gccaagaaga gttgaaaagg gcgatgaatt tgtcatgtaa
     2461 cttaatttgg gtcaatttga gcatgatgaa ttgaaatcat cccttgttgg gagttaataa
     2521 ccggtttgtt atcagaaacc ctgtaataga agggcgccct aactttgagc caattcatcc
     2581 cggtttctgt caaatatatc aaaaagtggt caactgacaa attgtttttg atattataat
     2641 aaacatttta tccgttaaca attttcgaat actttttaca aggacttgga taaattggct
     2701 caaagataac ttcgtataat gtatgctata cgaagttatt aactaatctg atttaaattt
     2761 tcaggaaccc ttccaatggg agttgttcgt ccacttaccg aggttgagat ggatcactac
     2821 cgcgagccat tccttaaccc agttgatcgc gagccacttt ggcgcttccc aaacgaactt
     2881 ccaatcgctg gagagccagc taacatcgtt gctcttgttg aggagtacat ggattggctt
     2941 caccaatctc cagttccaaa gcttcttttc tggggaactc caggagttct tatcccacca
     3001 gctgaggctg cccgtcttgc taagtctctt ccaaactgca aggctgtcga tatcggacca
     3061 ggacttaacc ttcttcaaga ggataaccca gatcttatcg gatctgagat cgctcgttgg
     3121 ctttctaccc ttgagatctc cggaggtcga agagc
//
