LOCUS       sequence-136597-        2999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Saptrap tagRFP + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS286
FEATURES             Location/Qualifiers
     source          1..2999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    556..589
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2553..2564
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2713..2746
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctcttcgat gatggttgtg tcgaaaggcg aagaactgat caaggaaaac atgcatatga
       61 aactgtacat ggaaggaacc gtcaataacc atcactttaa atgtacttcc gaaggagaag
      121 gtaaaccata cgaaggcacc cagactatgc gcattaaagt cgttgaagga ggtccactgc
      181 cgtttgcgtt cgatatcctg gccacctcat ttatgtacgg atctagaacc ttcatcaacc
      241 acactcaggg tatcccagat ttctttaaac aatcatttcc ggaaggattc acatgggaac
      301 gtgtgaccac ttatgaagat ggcggagtcc tgacagcaac gcaggatacg agcctgcaag
      361 atggttgcct gatttacaat gtcaaaatcc gcggtgttaa ttttccaagt aacggcccgg
      421 ttatgcagaa gaaaaccctg ggttgggaag caaacacgga aatgctgtat ccagctgatg
      481 gtggcctgga aggcagaact gatatggcac tgaaactggt gggaggtggg taagtttaaa
      541 catgatttta ctaacataac ttcgtataat gtatgctata cgaagttatt tctagacatt
      601 ctctaatgaa aaaatctttc agttgaaatt gaaaatgagt taaagttgga gtttttattg
      661 aaaacagatt tccgtgtgat tagtgttttt agcgagtgtg acaggacagc gaaaaaatat
      721 agaaacaagg ggggaactga aaagcttagg aatgcattga acatgagaag gggaagggga
      781 aggaacaaac tagacaggaa ttattggaat ttaatcacat ttggagtttt ttttctattc
      841 gacagaataa ttatccagaa catttttgta ttaaatattt atgcatcata tgagtagtcg
      901 gctttgttgt gcatgacgag tttgttatcg acgaaataga agctgtcaga acgagtctcg
      961 tttggattgt tgatcatgtc gtccactgaa aaagagatta gtcttttgaa ttgtactttt
     1021 tagaataatg actcactgag ttgttgagag agttgaggga actcatagat atgttcacag
     1081 ttgtttcgtg aattcggaat acagaatccg aattcaaagt caaaacactt cagaaggcga
     1141 tctttgaaga agtgacgttc gatcattcgg aaatgatgga ttgggatgtc tcctactttg
     1201 aattccacag ttgctccgac cgttttgagt ttcaaaaagt ttggagcaaa tctataacgc
     1261 acgtatcttg ccgactcctg tggcgactca tcattctcct gatcgttctc cggtttggcg
     1321 atctcgaaaa gcacttgctc agtgtccaga tcacggattt ggaacttggt gaactcgatg
     1381 ttatagatgt tcgcagatgg ggagcataag aatcctaaat ttatgtttta aactgaaatc
     1441 caaagggagc aagatacctt gagtgattcc cggaagtgct aaaacgtcgt tcggagtgat
     1501 ttgagctttc ttcgcaagct ccgattccgt tgtgattcct tgttcggtgc ttggtggtgg
     1561 ccgtggcatc tggaaatatg gaaaagttca acaaaaagaa aagagaaaag aatgaaatcg
     1621 gatatcaaga gttagttgag cggtttctct agttttctga gtctcacctg cgacgggaag
     1681 gtcgccgagc cgggtggaat cgattgttgt tgctcggctt tcatatcggt ttggttggaa
     1741 gcggctgaaa acggaaagaa gtggaagaag gaaaagagtg tggtgtgaca ggaaaatggt
     1801 aattagaggg tgccaaataa ccagctatat tttgtttttt tttgaaaaca tttttaaaaa
     1861 gaaaaatacg ataatgatat cagatggatt tccggaaaac tggtatgaaa aatttcaacc
     1921 tttttgagta catgtaatca aaatacactt tgtaaattat catttttatt gaaactccac
     1981 catttttcta tttataacgc taataatttg aaaaagaaac ctgttgcgaa ccgcggggtg
     2041 aatcccaaaa acgaatgcgt tttggtggag tgattgattc gaatcgaaga agaaaaagaa
     2101 gaagacgtgg aatagagagc tcactcttaa ccgagcagca cacaccgaca gaaaaaaaaa
     2161 tgaaatgaat gagggtcttc ttcttcttct tcttcgaatg attgacagaa atgggaaaaa
     2221 gaggaagatt gagaagggaa aaaggaagga gaaaagaagc agaagaagac gtcagagagg
     2281 agaggaacga gcggaaaagc agcgggcgca agtcatagaa gtagcagagc tggggagaag
     2341 aagacactat ccaagaaagg aatgacgaga gagtatgcaa aggggtatag ggtgcagaca
     2401 gaataggaac agaataacag atgatgagcc aagaagagtt gaaaagggcg atgaatttgt
     2461 catgtaactt aatttgggtc aatttgagca tgatgaattg aaatcatccc ttgttgggag
     2521 ttaataaccg gtttgttatc agaaaccctg taatagaagg gcgccctaac tttgagccaa
     2581 ttcatcccgg tttctgtcaa atatatcaaa aagtggtcaa ctgacaaatt gtttttgata
     2641 ttataataaa cattttatcc gttaacaatt ttcgaatact ttttacaagg acttggataa
     2701 attggctcaa agataacttc gtataatgta tgctatacga agttattaac taatctgatt
     2761 taaattttca gccatctgat ctgtaacttc aagacaacgt atcgttctaa aaaaccagcg
     2821 aaaaacctga aaatgccggg agtttattac gtggatcacc gtctggaacg catcaaagaa
     2881 gccgataaag aaacctatgt ggaacaacat gaagtggcag tcgctcgtta ctgcgatctg
     2941 ccgagcaaac tgggccacaa actgaatgga atggatgaac tgtacaaagg tcgaagagc
//
