LOCUS       sequence-136607-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS272
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..71
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    510..543
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcttcggc ggtaagttta aacatgattt tactaacgaa gttcctattc tctagaaagt
       61 ataggaactt ctaactaatc tgatttaaat tttcagggtt aaattttcaa attttaaata
      121 ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt
      181 tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg tctcccaatt
      241 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat
      301 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat
      361 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa
      421 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt
      481 aggcgaacga tgagaattgt cctcgcttgg aagttcctat tctctagaaa gtataggaac
      541 ttctaactaa tctgatttaa attttcaggg tagcggtggc agtggaggta ccggcggaag
      601 cggtatggga agagc
//
