LOCUS       sequence-136609-         503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on C-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS279
FEATURES             Location/Qualifiers
     source          1..503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..46
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    458..491
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcttcggc gggaagttcc tattctctag aaagtatagg aacttcataa attttcaaat
       61 tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt gtgttttttt
      121 tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc tctttccgtc
      181 tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat ttatcttctc
      241 gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca caaaaggatg
      301 atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa tatggaggaa
      361 ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa aaaataagca
      421 aagaaattag gcgaacgatg agaattgtcc tcgcttggaa gttcctattc tctagaaagt
      481 ataggaactt caatgggaag agc
//
