LOCUS       sequence-136615-         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS281
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    472..505
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcttcggg ttcggtaagt ttaaacatga ttttactaac gaagttccta ttctctagaa
       61 agtataggaa cttctaacta atctgattta aattttcagg gtagccatgg cttcccgccg
      121 gaggtggagg agcaggatga tggcacgctg cccatgtctt gtgcccagga gagcgggatg
      181 gaccgtcacc ctgcagcctg tgcttctgct aggatcaatg tgtaagctgt ctcatcctac
      241 tttcacctag ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc
      301 ctagaagctt acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc
      361 ttattgaatc tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta
      421 ttgtattttc aacttctaat tttaattcag ggaaactgct tcaacgcatc agaagttcct
      481 attctctaga aagtatagga acttctaact aatctgattt aaattttcag ggtatgggca
      541 gcggaggtac cggcggtagt ggaggcacgc gaagagc
//
