LOCUS       sequence-136617-         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap FLP-on N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS282
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..46
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    421..454
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcttcggg tggaagttcc tattctctag aaagtatagg aacttctagc catggcttcc
       61 cgccggaggt ggaggagcag gatgatggca cgctgcccat gtcttgtgcc caggagagcg
      121 ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtaa gctgtctcat
      181 cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta gtatctaaaa
      241 ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt gtatatttat
      301 tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt ccaattttct
      361 ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac gcatcaatgg
      421 gaagttccta ttctctagaa agtataggaa cttctacgcg aagagc
//
