LOCUS       sequence-136627-         230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Simple cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS280
FEATURES             Location/Qualifiers
     source          1..230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    55..162
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     71..87
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(122..138)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(122..138)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(175..195)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(175..193)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(214..230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(214..230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
       61 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      121 ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
      181 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
//
